A method for the radiohalogenation of proteins resulting in decreased thyroid uptake of radioiodine.

Journal Article (Journal Article)

A procedure is described for the radioiodination of proteins using an iodinated derivative of N-succinimidyl 3-(tri-n-butylstannyl) benzoate (ATE). Adequate removal of unreacted ATE from [125I]ATE was necessary for optimal protein radioiodination. Labeling efficiencies of greater than 60% could be obtained after a 20 min incubation of goat IgG with [125I]ATE at 4 degrees C. Paired-label experiments with goat IgG labeled with 125I using ATE and 131I using Iodogen demonstrated that use of the ATE reagent for protein labeling significantly reduced (P less than 0.005) the thyroid uptake of radioiodine.

Full Text

Duke Authors

Cited Authors

  • Zalutsky, MR; Narula, AS

Published Date

  • 1987

Published In

Volume / Issue

  • 38 / 12

Start / End Page

  • 1051 - 1055

PubMed ID

  • 2828275

International Standard Serial Number (ISSN)

  • 0883-2889

Digital Object Identifier (DOI)

  • 10.1016/0883-2889(87)90069-4


  • eng

Conference Location

  • England