Functional association of Nmi with Stat5 and Stat1 in IL-2- and IFNgamma-mediated signaling.

Published

Journal Article

Using the coiled-coil region of Stat5b as the bait in a yeast two-hybrid screen, we identified the association of Nmi, a protein of unknown function previously reported as an N-Myc interactor. We further show that Nmi interacts with all STATs except Stat2. We evaluated two cytokine systems, IL-2 and IFNgamma, and demonstrate that Nmi augments STAT-mediated transcription in response to these cytokines. Interestingly, Nmi lacks an intrinsic transcriptional activation domain; instead, Nmi enhances the association of CBP/p300 coactivator proteins with Stat1 and Stat5, and together with CBP/p300 can augment IL-2- and IFNgamma-dependent transcription. Therefore, our data not only reveal that Nmi can potentiate STAT-dependent transcription, but also suggest that it can augment coactivator protein recruitment to at least some members of a group of sequence-specific transcription factors.

Full Text

Duke Authors

Cited Authors

  • Zhu, M; John, S; Berg, M; Leonard, WJ

Published Date

  • January 8, 1999

Published In

Volume / Issue

  • 96 / 1

Start / End Page

  • 121 - 130

PubMed ID

  • 9989503

Pubmed Central ID

  • 9989503

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/s0092-8674(00)80965-4

Language

  • eng

Conference Location

  • United States