Butyrylcholinesterase activity in gestational diabetes: correlation with lymphocyte subpopulations in peripheral blood.

Published

Journal Article

PROBLEM: Inefficient clearance of pregnancy-threatening toxins may contribute to gestational diabetes (GD) and Type II diabetes mellitus (DM) through mechanisms involving immune dysregulation. METHOD OF STUDY: Peripheral venous blood from pregnant Kuwaiti women in third trimester, including 15 GD and 17 DM patients, 14 healthy pregnant (HP) and eight non-pregnant subjects, was analyzed by two-color flow cytometery for number and percentage representation of T lymphocytes. Buterylcholinesterase (BuChE) activity was measured using buterylthiocholine iodide and spectrophotometry. RESULTS: Relative to HP, GD patients exhibited higher ratios of activated and memory phenotypes, including CD4+ CD25+ (P < 0.01), CD4+ HLA-DR (P < 0.05) and CD4+ CD45RO+ (P < 0.05) cells. Serum BuChE activity exhibited positive correlation within the HP cohort with CD4+ CD25+ (P < 0.05), but not in GD and DM cohorts. CONCLUSIONS: Positive correlation between BuChE and a (presumptive) 'regulatory' T-cell phenotype in HP, but not GD or DM may indicate existence of protective detoxification mechanisms against oxidative stress in normal pregnancies.

Full Text

Duke Authors

Cited Authors

  • Mahmoud, FF; Haines, DD; Abul, HT; Omu, AE; Abu-Donia, MB

Published Date

  • September 2006

Published In

Volume / Issue

  • 56 / 3

Start / End Page

  • 185 - 192

PubMed ID

  • 16911714

Pubmed Central ID

  • 16911714

International Standard Serial Number (ISSN)

  • 1046-7408

Digital Object Identifier (DOI)

  • 10.1111/j.1600-0897.2006.00416.x

Language

  • eng

Conference Location

  • Denmark