Combined exposure to sarin and pyridostigmine bromide increased levels of rat urinary 3-nitrotyrosine and 8-hydroxy-2'-deoxyguanosine, biomarkers of oxidative stress.

Journal Article

In this study concentrations of markers of oxidative stress 3-nitrotyrosine and 8-hydroxy-2'-deoxyguanosine (8-OhdG) were determined in rat urine following a single oral dose of pyridostigmine bromide (PB) 13 mg/kg and a single intramuscular dose of sarin 80 microg/kg alone or in combination. Urine samples were collected 16, 24, 48, 72, and 96 h following dosing. Control urine samples of five rats treated with normal saline were also collected at the same time intervals. A combined dose of PB and sarin significantly increased levels of 3-nitrotyrosine and (8-OhdG) starting 48 h after dosing. An increase in the concentration of these markers was not detected following a single dose of PB or sarin alone. Maximal increase in 3-nitrotyrosine and 8-OhdG was detected 48 h after administration of a combination PB and sarin. The results indicate that concurrent exposure to PB and sarin could generate free radical species that may cause oxidative stress in rats. The results may have significant impact if veterans were expose to sarin following an oral dose of PB.

Full Text

Duke Authors

Cited Authors

  • Abu-Qare, AW; Abou-Donia, MB

Published Date

  • August 6, 2001

Published In

Volume / Issue

  • 123 / 1

Start / End Page

  • 51 - 58

PubMed ID

  • 11514105

International Standard Serial Number (ISSN)

  • 0378-4274

Language

  • eng

Conference Location

  • Netherlands