Combined exposure to DEET (N,N-diethyl-m-toluamide) and permethrin-induced release of rat brain mitochondrial cytochrome c.


Journal Article

The release of cytochrome c from the mitochondrial intermembrane space can induce apoptosis. The levels of mitochondrial cytochrome c in rat brain following a single dermal dose of 400 mg/kg of DEET, and of 1.3 mg/kg of permethrin, alone or in combination were determined. Rats were sacrificed at a time interval of 0.5, 1, 2, 4, 8, 16, 24, 48, or 72 h after dosing. Brain mitochondria were isolated and the levels of cytochrome c were measured using reversed-phase high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. Average percentage recovery of cytochrome c spiked with control rat brain mitochondria was 83.2 +/- 8.9%. Limits of detection and quantitation were 1 and 5 ng, respectively. The results showed that a single dermal dose of a combination of DEET and permethrin significantly increased the release of brain mitochondrial cytochrome c starting 24 h after treatment. DEET and permethrin alone did not affect the release of cytochrome c. The results indicate that combined exposure to DEET and permethrin might induce the apoptotic processes in rat brain as seen by the release of cytochrome c.

Full Text

Duke Authors

Cited Authors

  • Abu-Qare, AW; Abou-Donia, MB

Published Date

  • June 22, 2001

Published In

Volume / Issue

  • 63 / 4

Start / End Page

  • 243 - 252

PubMed ID

  • 11437058

Pubmed Central ID

  • 11437058

International Standard Serial Number (ISSN)

  • 1528-7394

Digital Object Identifier (DOI)

  • 10.1080/15287390151143640


  • eng

Conference Location

  • England