P-glycoprotein involvement in cuticular penetration of [14C]thiodicarb in resistant tobacco budworms.


Journal Article

Pesticides have been shown to interact with the multidrug resistance protein associated with cancer chemotherapy, P-glycoprotein (P-gp). P-gp, therefore, has also been implicated in the development of pesticide resistance. The purpose of this study was to characterize the effect P-gp has on the accumulation of the carbamate pesticide, thiodicarb. For these studies, resistant tobacco budworm larvae, expressing four times the P-gp as susceptible larvae, were pretreated with the P-gp inhibitor, quinidine, and challenged topically with thiodicarb. Quinidine enhanced thiodicarb toxicity in a dose-dependent manner, with mortality in the presence of P-gp inhibition increased up to 33%. Quinidine treatment increased [14C]thiodicarb accumulation 2- to 3-fold as compared to thiodicarb treatment alone. This study suggests that P-gp contributes to quinidine synergism of thiodicarb toxicity and suggests that P-gp may be involved in cuticular resistance to pesticides.

Full Text

Duke Authors

Cited Authors

  • Lanning, CL; Ayad, HM; Abou-Donia, MB

Published Date

  • June 1996

Published In

Volume / Issue

  • 85 / 3

Start / End Page

  • 127 - 133

PubMed ID

  • 8644124

Pubmed Central ID

  • 8644124

International Standard Serial Number (ISSN)

  • 0378-4274

Digital Object Identifier (DOI)

  • 10.1016/0378-4274(96)03654-5


  • eng

Conference Location

  • Netherlands