The effects of tri-o-cresyl phosphate and metabolites on rat Sertoli cell function in primary culture.


Journal Article

A neurotoxic organophosphate, tri-o-cresyl phosphate (TOCP) is also a testicular toxicant. Histopathologic damage in the testis is first seen in Sertoli cells. TOCP and its activated metabolite saligenin cyclic-o-tolyl phosphate (SCOTP) were evaluated for effects on rat Sertoli cells in primary culture. SCOTP, but not TOCP, caused minor morphologic effects on the cells and increased levels of lactate in the spent medium with no change in pyruvate levels, synthesis of cellular or secreted proteins, or the cyclic AMP response to FSH stimulation. SCOTP was the metabolite of TOCP that produced the largest decrease in nonspecific esterase activity in Sertoli cells (up to 80%), when tested in the concentration range found in vivo. This decrease is consistent with previous in vivo evidence. These in vitro experiments replicate previously observed in vivo biochemical effects and suggest that SCOTP is the metabolite responsible for at least some of the biochemical effects seen in the testis after TOCP exposure.

Full Text

Duke Authors

Cited Authors

  • Chapin, RE; Phelps, JL; Burka, LT; Abou-Donia, MB; Heindel, JJ

Published Date

  • April 1991

Published In

Volume / Issue

  • 108 / 2

Start / End Page

  • 194 - 204

PubMed ID

  • 1902005

Pubmed Central ID

  • 1902005

International Standard Serial Number (ISSN)

  • 0041-008X

Digital Object Identifier (DOI)

  • 10.1016/0041-008x(91)90110-z


  • eng

Conference Location

  • United States