Calcium and calmodulin stimulated in vitro phosphorylation of rooster brain tubulin and MAP-2 following a single oral dose of tri-o-cresyl phosphate.


Journal Article

The effect of a single oral administration of 750 mg/kg tri-o-cresyl phosphate (TOCP) on endogenous phosphorylation of specific brain cytosolic proteins has been studied in roosters following the development of delayed neurotoxicity. In vitro phosphorylation assay using [gamma-32P]ATP was carried out. Proteins were then resolved on one dimensional 8% SDS-PAGE and two-dimensional gel electrophoresis, stained with Coomassie blue and autoradiographed. The amount of proteins, as well as the amount of 32P incorporation, were quantified by microdensitometry, TOCP-administration enhanced the phosphorylation of cytosolic proteins of M, 52-59 kDa, 70 kDa, and 300 kDa by as much as 155%, 199% and 166%, respectively. Two-dimensional gel electrophoresis confirmed the 52-59 kDa proteins as alpha and beta tubulin and the 300 kDa protein as microtubule-associated protein-2.

Full Text

Duke Authors

Cited Authors

  • Suwita, E; Lapadula, DM; Abou-Donia, MB

Published Date

  • May 21, 1986

Published In

Volume / Issue

  • 374 / 1

Start / End Page

  • 199 - 203

PubMed ID

  • 3719328

Pubmed Central ID

  • 3719328

International Standard Serial Number (ISSN)

  • 0006-8993

Digital Object Identifier (DOI)

  • 10.1016/0006-8993(86)90412-9


  • eng

Conference Location

  • Netherlands