Skip to main content

Synthetic mast-cell granules as adjuvants to promote and polarize immunity in lymph nodes.

Publication ,  Journal Article
St John, AL; Chan, CY; Staats, HF; Leong, KW; Abraham, SN
Published in: Nat Mater
January 22, 2012

Granules of mast cells (MCs) enhance adaptive immunity when, on activation, they are released as stable particles. Here we show that submicrometre particles modelled after MC granules augment immunity when used as adjuvants in vaccines. The synthetic particles, which consist of a carbohydrate backbone with encapsulated inflammatory mediators such as tumour necrosis factor, replicate attributes of MCs in vivo including the targeting of draining lymph nodes and the timed release of the encapsulated mediators. When used as an adjuvant during vaccination of mice with haemagglutinin from the influenza virus, the particles enhanced adaptive immune responses and increased survival of mice on lethal challenge. Furthermore, differential loading of the particles with the cytokine IL-12 directed the character of the response towards Th1 lymphocytes. The synthetic MC adjuvants replicate and enhance the functions of MCs during vaccination, and can be extended to polarize the resulting immunity.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Nat Mater

DOI

EISSN

1476-4660

Publication Date

January 22, 2012

Volume

11

Issue

3

Start / End Page

250 / 257

Location

England

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Nanoscience & Nanotechnology
  • Mice, Inbred BALB C
  • Mice
  • Mast Cells
  • Lymph Nodes
  • Interleukin-12
  • Female
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
St John, A. L., Chan, C. Y., Staats, H. F., Leong, K. W., & Abraham, S. N. (2012). Synthetic mast-cell granules as adjuvants to promote and polarize immunity in lymph nodes. Nat Mater, 11(3), 250–257. https://doi.org/10.1038/nmat3222
St John, Ashley L., Cheryl Y. Chan, Herman F. Staats, Kam W. Leong, and Soman N. Abraham. “Synthetic mast-cell granules as adjuvants to promote and polarize immunity in lymph nodes.Nat Mater 11, no. 3 (January 22, 2012): 250–57. https://doi.org/10.1038/nmat3222.
St John AL, Chan CY, Staats HF, Leong KW, Abraham SN. Synthetic mast-cell granules as adjuvants to promote and polarize immunity in lymph nodes. Nat Mater. 2012 Jan 22;11(3):250–7.
St John, Ashley L., et al. “Synthetic mast-cell granules as adjuvants to promote and polarize immunity in lymph nodes.Nat Mater, vol. 11, no. 3, Jan. 2012, pp. 250–57. Pubmed, doi:10.1038/nmat3222.
St John AL, Chan CY, Staats HF, Leong KW, Abraham SN. Synthetic mast-cell granules as adjuvants to promote and polarize immunity in lymph nodes. Nat Mater. 2012 Jan 22;11(3):250–257.

Published In

Nat Mater

DOI

EISSN

1476-4660

Publication Date

January 22, 2012

Volume

11

Issue

3

Start / End Page

250 / 257

Location

England

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Nanoscience & Nanotechnology
  • Mice, Inbred BALB C
  • Mice
  • Mast Cells
  • Lymph Nodes
  • Interleukin-12
  • Female
  • Animals