Evidence for an immune barrier after in utero hematopoietic-cell transplantation.
The competence of the immune system of the developing fetus to act as a barrier to in utero hematopoietic-cell transplantation (IUHCT) has been a source of debate. Until now, comparisons of allogeneic and congenic engraftment have been inconclusive due to methodologic limitations resulting in minimal and inefficient engraftment. In this study, E14 fetal mice received transplants of either allogeneic or congenic bone marrow using a new intravascular technique that allows definitive administration of much higher doses of donor cells. Our results demonstrate that 100% of surviving recipients demonstrate engraftment at 1 week of age, but that 70% of allogeneic recipients lose engraftment by 1 month of age, and 80% ultimately fail to sustain long-term chimerism. In contrast, all congenic recipients maintain stable, long-term, multilineage chimerism. These results strongly support an immune barrier to allogeneic engraftment after IUHCT.
Duke Scholars
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- Transplantation, Isogeneic
- Transplantation, Homologous
- Transplantation Immunology
- Survival Rate
- Pregnancy
- Mice, Inbred Strains
- Mice
- Immunology
- Immunity
- Hematopoietic Stem Cell Transplantation
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transplantation, Isogeneic
- Transplantation, Homologous
- Transplantation Immunology
- Survival Rate
- Pregnancy
- Mice, Inbred Strains
- Mice
- Immunology
- Immunity
- Hematopoietic Stem Cell Transplantation