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Programming the magnitude and persistence of antibody responses with innate immunity.

Publication ,  Journal Article
Kasturi, SP; Skountzou, I; Albrecht, RA; Koutsonanos, D; Hua, T; Nakaya, HI; Ravindran, R; Stewart, S; Alam, M; Kwissa, M; Villinger, F ...
Published in: Nature
February 24, 2011

Many successful vaccines induce persistent antibody responses that can last a lifetime. The mechanisms by which they do so remain unclear, but emerging evidence indicates that they activate dendritic cells via Toll-like receptors (TLRs). For example, the yellow fever vaccine YF-17D, one of the most successful empiric vaccines ever developed, activates dendritic cells via multiple TLRs to stimulate proinflammatory cytokines. Triggering specific combinations of TLRs in dendritic cells can induce synergistic production of cytokines, which results in enhanced T-cell responses, but its impact on antibody responses remain unknown. Learning the critical parameters of innate immunity that program such antibody responses remains a major challenge in vaccinology. Here we demonstrate that immunization of mice with synthetic nanoparticles containing antigens plus ligands that signal through TLR4 and TLR7 induces synergistic increases in antigen-specific, neutralizing antibodies compared to immunization with nanoparticles containing antigens plus a single TLR ligand. Consistent with this there was enhanced persistence of germinal centres and of plasma-cell responses, which persisted in the lymph nodes for >1.5 years. Surprisingly, there was no enhancement of the early short-lived plasma-cell response relative to that observed with single TLR ligands. Molecular profiling of activated B cells, isolated 7 days after immunization, indicated that there was early programming towards B-cell memory. Antibody responses were dependent on direct triggering of both TLRs on B cells and dendritic cells, as well as on T-cell help. Immunization protected completely against lethal avian and swine influenza virus strains in mice, and induced robust immunity against pandemic H1N1 influenza in rhesus macaques.

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Published In

Nature

DOI

EISSN

1476-4687

Publication Date

February 24, 2011

Volume

470

Issue

7335

Start / End Page

543 / 547

Location

England

Related Subject Headings

  • Toll-Like Receptors
  • T-Lymphocytes
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Plasma Cells
  • Nanoparticles
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Macaca mulatta
 

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Kasturi, S. P., Skountzou, I., Albrecht, R. A., Koutsonanos, D., Hua, T., Nakaya, H. I., … Pulendran, B. (2011). Programming the magnitude and persistence of antibody responses with innate immunity. Nature, 470(7335), 543–547. https://doi.org/10.1038/nature09737
Kasturi, Sudhir Pai, Ioanna Skountzou, Randy A. Albrecht, Dimitrios Koutsonanos, Tang Hua, Helder I. Nakaya, Rajesh Ravindran, et al. “Programming the magnitude and persistence of antibody responses with innate immunity.Nature 470, no. 7335 (February 24, 2011): 543–47. https://doi.org/10.1038/nature09737.
Kasturi SP, Skountzou I, Albrecht RA, Koutsonanos D, Hua T, Nakaya HI, et al. Programming the magnitude and persistence of antibody responses with innate immunity. Nature. 2011 Feb 24;470(7335):543–7.
Kasturi, Sudhir Pai, et al. “Programming the magnitude and persistence of antibody responses with innate immunity.Nature, vol. 470, no. 7335, Feb. 2011, pp. 543–47. Pubmed, doi:10.1038/nature09737.
Kasturi SP, Skountzou I, Albrecht RA, Koutsonanos D, Hua T, Nakaya HI, Ravindran R, Stewart S, Alam M, Kwissa M, Villinger F, Murthy N, Steel J, Jacob J, Hogan RJ, García-Sastre A, Compans R, Pulendran B. Programming the magnitude and persistence of antibody responses with innate immunity. Nature. 2011 Feb 24;470(7335):543–547.
Journal cover image

Published In

Nature

DOI

EISSN

1476-4687

Publication Date

February 24, 2011

Volume

470

Issue

7335

Start / End Page

543 / 547

Location

England

Related Subject Headings

  • Toll-Like Receptors
  • T-Lymphocytes
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Plasma Cells
  • Nanoparticles
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Macaca mulatta