Low prevalence of myocilin mutations in an African American population with primary open-angle glaucoma.

Journal Article (Journal Article)

PURPOSE: Mutations in the myocilin gene (MYOC) are associated with primary open-angle glaucoma (POAG) in many different populations. This study represents the first large survey of MYOC mutations in an African American population. METHODS: We recruited 529 African American subjects with POAG and 270 African American control subjects in this study. A complete eye examination and blood collection was performed in all study subjects. Genomic DNA was extracted. The entire coding sequence of MYOC was amplified and sequenced using the Sanger method. Identified MYOC variants were compared with previously reported MYOC mutations. RESULTS: We identified a total of 29 MYOC variants including six potential MYOC mutations. Two mutations (Thr209Asn and Leu215Gln) are novel and are found only in cases and no controls. We also identified four previously reported MYOC mutations in cases and no controls (Tyr453MetfsX11, Gln368X, Thr377Met, and Ser393Arg). The overall frequency of glaucoma-causing MYOC mutations in our African American population with POAG was 1.4%. CONCLUSIONS: We identified two novel probable glaucoma-causing MYOC mutations (Thr209Asn and Leu215Gln). This study indicates that, despite the high prevalence of POAG, MYOC mutations are rare in the African American population.

Full Text

Duke Authors

Cited Authors

  • Liu, W; Liu, Y; Challa, P; Herndon, LW; Wiggs, JL; Girkin, CA; Allingham, RR; Hauser, MA

Published Date

  • 2012

Published In

Volume / Issue

  • 18 /

Start / End Page

  • 2241 - 2246

PubMed ID

  • 22933836

Pubmed Central ID

  • PMC3429360

Electronic International Standard Serial Number (EISSN)

  • 1090-0535


  • eng

Conference Location

  • United States