Biochemical characterization of the Ca2+ release channel of skeletal and cardiac sarcoplasmic reticulum.

Journal Article (Journal Article)

Rapid mixing-vesicle ion flux and planar lipid bilayer-single channel measurements have shown that a high-conductance, ligand-gated Ca2+ release channel is present in 'heavy', junctional-derived membrane fractions of skeletal and cardiac muscle sarcoplasmic reticulum. Using the release channel-specific probe, ryanodine, a 30S protein complex composed of polypeptides of Mr approximately 400,000 has been isolated from cardiac and skeletal muscle. Reconstitution of the complex into planar lipid bilayers has revealed a Ca2+ conductance with properties characteristic of the native Ca2+ release channel.

Full Text

Duke Authors

Cited Authors

  • Meissner, G; Rousseau, E; Lai, FA; Liu, QY; Anderson, KA

Published Date

  • 1988

Published In

Volume / Issue

  • 82 / 1-2

Start / End Page

  • 59 - 65

PubMed ID

  • 2847014

International Standard Serial Number (ISSN)

  • 0300-8177

Digital Object Identifier (DOI)

  • 10.1007/BF00242517


  • eng

Conference Location

  • Netherlands