Scara5 is a ferritin receptor mediating non-transferrin iron delivery.

Published

Journal Article

Developing organs require iron for a myriad of functions, but embryos deleted of the major adult transport proteins, transferrin or its receptor transferrin receptor1 (TfR1(-/-)), still initiate organogenesis, suggesting that non-transferrin pathways are important. To examine these pathways, we developed chimeras composed of fluorescence-tagged TfR1(-/-) cells and untagged wild-type cells. In the kidney, TfR1(-/-) cells populated capsule and stroma, mesenchyme and nephron, but were underrepresented in ureteric bud tips. Consistently, TfR1 provided transferrin to the ureteric bud, but not to the capsule or the stroma. Instead of transferrin, we found that the capsule internalized ferritin. Since the capsule expressed a novel receptor called Scara5, we tested its role in ferritin uptake and found that Scara5 bound serum ferritin and then stimulated its endocytosis from the cell surface with consequent iron delivery. These data implicate cell type-specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways.

Full Text

Duke Authors

Cited Authors

  • Li, JY; Paragas, N; Ned, RM; Qiu, A; Viltard, M; Leete, T; Drexler, IR; Chen, X; Sanna-Cherchi, S; Mohammed, F; Williams, D; Lin, CS; Schmidt-Ott, KM; Andrews, NC; Barasch, J

Published Date

  • January 2009

Published In

Volume / Issue

  • 16 / 1

Start / End Page

  • 35 - 46

PubMed ID

  • 19154717

Pubmed Central ID

  • 19154717

Electronic International Standard Serial Number (EISSN)

  • 1878-1551

International Standard Serial Number (ISSN)

  • 1534-5807

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2008.12.002

Language

  • eng