Transferrin receptor 1 is a cellular receptor for New World haemorrhagic fever arenaviruses.

Published

Journal Article

At least five arenaviruses cause viral haemorrhagic fevers in humans. Lassa virus, an Old World arenavirus, uses the cellular receptor alpha-dystroglycan to infect cells. Machupo, Guanarito, Junin and Sabia viruses are New World haemorrhagic fever viruses that do not use alpha-dystroglycan. Here we show a specific, high-affinity association between transferrin receptor 1 (TfR1) and the entry glycoprotein (GP) of Machupo virus. Expression of human TfR1, but not human transferrin receptor 2, in hamster cell lines markedly enhanced the infection of viruses pseudotyped with the GP of Machupo, Guanarito and Junin viruses, but not with those of Lassa or lymphocytic choriomeningitis viruses. An anti-TfR1 antibody efficiently inhibited the replication of Machupo, Guanarito, Junin and Sabia viruses, but not that of Lassa virus. Iron depletion of culture medium enhanced, and iron supplementation decreased, the efficiency of infection by Junin and Machupo but not Lassa pseudoviruses. These data indicate that TfR1 is a cellular receptor for New World haemorrhagic fever arenaviruses.

Full Text

Duke Authors

Cited Authors

  • Radoshitzky, SR; Abraham, J; Spiropoulou, CF; Kuhn, JH; Nguyen, D; Li, W; Nagel, J; Schmidt, PJ; Nunberg, JH; Andrews, NC; Farzan, M; Choe, H

Published Date

  • March 2007

Published In

Volume / Issue

  • 446 / 7131

Start / End Page

  • 92 - 96

PubMed ID

  • 17287727

Pubmed Central ID

  • 17287727

Electronic International Standard Serial Number (EISSN)

  • 1476-4687

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/nature05539

Language

  • eng