A mutation in Sec15l1 causes anemia in hemoglobin deficit (hbd) mice.

Published

Journal Article

Hemoglobin deficit (hbd) mice carry a spontaneous mutation that impairs erythroid iron assimilation but does not cause other defects. Normal delivery of iron to developing erythroid precursors is highly dependent on the transferrin cycle. Through genetic mapping and complementation experiments, we show that the hbd mutation is an in-frame deletion of a conserved exon of the mouse gene Sec15l1, encoding one of two Sec15 proteins implicated in the mammalian exocyst complex. Sec15l1 is linked to the transferrin cycle through its interaction with Rab11, a GTPase involved in vesicular trafficking. We propose that inactivation of Sec15l1 alters recycling of transferrin cycle endosomes and increases the release of transferrin receptor exocytic vesicles. This in turn decreases erythroid iron uptake. Determining the molecular basis of the hbd phenotype provides new insight into the intricate mechanisms necessary for normal erythroid iron uptake and the function of a mammalian exocyst protein.

Full Text

Duke Authors

Cited Authors

  • Lim, JE; Jin, O; Bennett, C; Morgan, K; Wang, F; Trenor, CC; Fleming, MD; Andrews, NC

Published Date

  • November 2005

Published In

Volume / Issue

  • 37 / 11

Start / End Page

  • 1270 - 1273

PubMed ID

  • 16227995

Pubmed Central ID

  • 16227995

International Standard Serial Number (ISSN)

  • 1061-4036

Digital Object Identifier (DOI)

  • 10.1038/ng1659

Language

  • eng

Conference Location

  • United States