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cAMP-dependent protein kinase is necessary for increased NF-E2.DNA complex formation during erythroleukemia cell differentiation.

Publication ,  Journal Article
Garingo, AD; Suhasini, M; Andrews, NC; Pilz, RB
Published in: J Biol Chem
April 21, 1995

When murine erythroleukemia (MEL) cells are induced to differentiate by hexamethylene bisacetamide (HMBA), erythroid-specific genes are transcriptionally activated; however, transcriptional activation of these genes is severely impaired in cAMP-dependent protein kinase (protein kinase A)-deficient MEL cells. The transcription factor NF-E2, composed of a 45-kDa (p45) and an 18-kDa (p18) subunit, is essential for enhancer activity of the globin locus control regions (LCRs). DNA binding of NF-E2 and alpha-globin LCR enhancer activity was significantly less in HMBA-treated protein kinase A-deficient cells compared to cells containing normal protein kinase A activity; DNA binding of several other transcription factors was the same in both cell types. In parental cells, HMBA treatment and/or prolonged activation of protein kinase A increased the amount of NF-E2.DNA complexes without change in DNA binding affinity; the expression of p45 and p18 was the same under all conditions. p45 and p18 were phosphorylated by protein kinase A in vitro, but the phosphorylation did not affect NF-E2.DNA complexes, suggesting that protein kinase A regulates NF-E2.DNA complex formation indirectly, e.g. by altering expression of a regulatory factor(s). Thus, protein kinase A appears to be necessary for increased NF-E2.DNA complex formation during differentiation of MEL cells and may influence erythroid-specific gene expression through this mechanism.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

April 21, 1995

Volume

270

Issue

16

Start / End Page

9169 / 9177

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Transcription Factors
  • Promoter Regions, Genetic
  • Phosphorylation
  • NF-E2 Transcription Factor, p45 Subunit
  • NF-E2 Transcription Factor
  • Molecular Sequence Data
  • Leukemia, Erythroblastic, Acute
  • Erythroid-Specific DNA-Binding Factors
 

Citation

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Garingo, A. D., Suhasini, M., Andrews, N. C., & Pilz, R. B. (1995). cAMP-dependent protein kinase is necessary for increased NF-E2.DNA complex formation during erythroleukemia cell differentiation. J Biol Chem, 270(16), 9169–9177. https://doi.org/10.1074/jbc.270.16.9169
Garingo, A. D., M. Suhasini, N. C. Andrews, and R. B. Pilz. “cAMP-dependent protein kinase is necessary for increased NF-E2.DNA complex formation during erythroleukemia cell differentiation.J Biol Chem 270, no. 16 (April 21, 1995): 9169–77. https://doi.org/10.1074/jbc.270.16.9169.
Garingo AD, Suhasini M, Andrews NC, Pilz RB. cAMP-dependent protein kinase is necessary for increased NF-E2.DNA complex formation during erythroleukemia cell differentiation. J Biol Chem. 1995 Apr 21;270(16):9169–77.
Garingo, A. D., et al. “cAMP-dependent protein kinase is necessary for increased NF-E2.DNA complex formation during erythroleukemia cell differentiation.J Biol Chem, vol. 270, no. 16, Apr. 1995, pp. 9169–77. Pubmed, doi:10.1074/jbc.270.16.9169.
Garingo AD, Suhasini M, Andrews NC, Pilz RB. cAMP-dependent protein kinase is necessary for increased NF-E2.DNA complex formation during erythroleukemia cell differentiation. J Biol Chem. 1995 Apr 21;270(16):9169–9177.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

April 21, 1995

Volume

270

Issue

16

Start / End Page

9169 / 9177

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Transcription Factors
  • Promoter Regions, Genetic
  • Phosphorylation
  • NF-E2 Transcription Factor, p45 Subunit
  • NF-E2 Transcription Factor
  • Molecular Sequence Data
  • Leukemia, Erythroblastic, Acute
  • Erythroid-Specific DNA-Binding Factors