Microcytic anemia in mk/mk mice is not corrected by retroviral-mediated gene transfer of wild-type p45 NF-E2.

Published

Journal Article

Mice homozygous for the mk mutation have a severe hypochromic, microcytic anemia that is characterized by a decreased mean-corpuscular hemoglobin concentration and balanced alpha- and beta-globin-chain synthesis. Transplantation studies have shown that the defect in homozygous mk/mk mice is intrinsic to both the hematopoietic system and the gut. The gene for the hematopoietic-specific transcription factor, p45 NF-E2, has been found to cosegregate with the mk phenotype and contain a point mutation in mk/mk mice that results in an amino acid substitution (173V-->A). In order to test the hypothesis that this amino acid substitution is responsible for the mk phenotype, we have used recombinant retroviruses to introduce wild-type p45 NF-E2 into the bone marrow of mk/mk mice. Despite gene transfer and expression of p45 NF-E2 in erythroid cells, we found no evidence for correction of the phenotype in mk/mk mice. These results indicate that the mk mutation cannot be corrected by enforced expression of wild-type p45 NF-E2 and suggest that the 173V-->A mutation of the p45 NF-E2 gene is not the cause of anemia in mk/mk mice.

Full Text

Duke Authors

Cited Authors

  • Ney, PA; Farina, SF; Bodine, DM; Andrews, NC; Orkin, SH; Neinhuis, AW

Published Date

  • January 1995

Published In

Volume / Issue

  • 23 / 1

Start / End Page

  • 74 - 80

PubMed ID

  • 7995373

Pubmed Central ID

  • 7995373

Electronic International Standard Serial Number (EISSN)

  • 1873-2399

International Standard Serial Number (ISSN)

  • 0301-472X

Language

  • eng