Continuous human glioma-derived cell lines UC-11MG and UC-302MG. Morphologic, immunocytochemical and chromosomal characterization.

Journal Article (Journal Article)

Two continuous human glioma-derived cell lines, UC-11MG and UC-302MG were established in our laboratory. Both cell lines persistently showed cytologic features similar to those of their respective original tumors. UC-11MG expressed glial fibrillary acidic protein (GFAP) and S-100 protein. The cell lines were negative for factor VIII related antigen (FVIII/RAg) and positive for fibronectin and neuronal specific enolase (NSE). Electron microscopic studies of UC-11MG revealed intermediate filament; filopodia and pinocytic vesicles were present in both lines. Dibutyryl cAMP (dB-cAMP) caused inhibition of growth and marked shift in the morphology of UC-302MG toward spindle cells. The cytologic appearance of UC-11MG treated with dB-cAMP was altered less, but cells showed a stronger GFAP expression, with 'cable' formation. Doubling time was 41.0 +/- 6.4 hours for UC-11MG and 43.7 +/- 6.6 hours for UC-302MG. The karyotypes of both cell lines were aneuploid with chromosomal derangement and markers characteristic for each line.

Full Text

Duke Authors

Cited Authors

  • Liwnicz, BH; Archer, G; Soukup, SW; Liwnicz, RG

Published Date

  • 1986

Published In

Volume / Issue

  • 3 / 4

Start / End Page

  • 373 - 385

PubMed ID

  • 3007688

International Standard Serial Number (ISSN)

  • 0167-594X

Digital Object Identifier (DOI)

  • 10.1007/BF00165588


  • eng

Conference Location

  • United States