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Platelet factor 4/heparin antibodies in blood bank donors.

Publication ,  Journal Article
Hursting, MJ; Pai, PJ; McCracken, JE; Hwang, F; Suvarna, S; Lokhnygina, Y; Bandarenko, N; Arepally, GM
Published in: Am J Clin Pathol
November 2010

Platelet factor 4 (PF4)/heparin antibody, typically associated with heparin therapy, is reported in some heparin-naive people. Seroprevalence in the general population, however, remains unclear. We prospectively evaluated PF4/heparin antibody in approximately 4,000 blood bank donors using a commercial enzyme-linked immunosorbent assay for initial and then repeated (confirmatory) testing. Antibody was detected initially in 249 (6.6%; 95% confidence interval [CI], 5.8%-7.4%) of 3,795 donors and repeatedly in 163 (4.3%; 95% CI, 3.7%-5.0%) of 3,789 evaluable donors. "Unconfirmed" positives were mostly (93%) low positives (optical density [OD] = 0.40-0.59). Of 163 repeatedly positive samples, 116 (71.2%) were low positives, and 124 (76.1%) exhibited heparin-dependent binding. Predominant isotypes of intermediate to high seropositive samples (OD >0.6) were IgG (20/39 [51%]), IgM (9/39 [23%]), and indeterminate (10/39 [26%]). The marked background seroprevalence of PF4/heparin antibody (4.3%-6.6%) with the preponderance of low (and frequently nonreproducible) positives in blood donors suggests the need for further assay calibration, categorization of antibody level, and studies evaluating clinical relevance of "naturally occurring" PF4/heparin antibodies.

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Published In

Am J Clin Pathol

DOI

EISSN

1943-7722

Publication Date

November 2010

Volume

134

Issue

5

Start / End Page

774 / 780

Location

England

Related Subject Headings

  • Seroepidemiologic Studies
  • Platelet Factor 4
  • Pathology
  • Linear Models
  • Humans
  • Heparin
  • Enzyme-Linked Immunosorbent Assay
  • Blood Donors
  • Antibodies
  • 3202 Clinical sciences
 

Citation

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Hursting, M. J., Pai, P. J., McCracken, J. E., Hwang, F., Suvarna, S., Lokhnygina, Y., … Arepally, G. M. (2010). Platelet factor 4/heparin antibodies in blood bank donors. Am J Clin Pathol, 134(5), 774–780. https://doi.org/10.1309/AJCPG0MNR5NGKNFX
Hursting, Marcie J., Poulomi J. Pai, Julianna E. McCracken, Fred Hwang, Shayela Suvarna, Yuliya Lokhnygina, Nicholas Bandarenko, and Gowthami M. Arepally. “Platelet factor 4/heparin antibodies in blood bank donors.Am J Clin Pathol 134, no. 5 (November 2010): 774–80. https://doi.org/10.1309/AJCPG0MNR5NGKNFX.
Hursting MJ, Pai PJ, McCracken JE, Hwang F, Suvarna S, Lokhnygina Y, et al. Platelet factor 4/heparin antibodies in blood bank donors. Am J Clin Pathol. 2010 Nov;134(5):774–80.
Hursting, Marcie J., et al. “Platelet factor 4/heparin antibodies in blood bank donors.Am J Clin Pathol, vol. 134, no. 5, Nov. 2010, pp. 774–80. Pubmed, doi:10.1309/AJCPG0MNR5NGKNFX.
Hursting MJ, Pai PJ, McCracken JE, Hwang F, Suvarna S, Lokhnygina Y, Bandarenko N, Arepally GM. Platelet factor 4/heparin antibodies in blood bank donors. Am J Clin Pathol. 2010 Nov;134(5):774–780.
Journal cover image

Published In

Am J Clin Pathol

DOI

EISSN

1943-7722

Publication Date

November 2010

Volume

134

Issue

5

Start / End Page

774 / 780

Location

England

Related Subject Headings

  • Seroepidemiologic Studies
  • Platelet Factor 4
  • Pathology
  • Linear Models
  • Humans
  • Heparin
  • Enzyme-Linked Immunosorbent Assay
  • Blood Donors
  • Antibodies
  • 3202 Clinical sciences