Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin.

Published

Journal Article

We have determined the nucleotide sequence of four submaxillary gland mRNAs, designated PS, S1, S2, and S3, that encode kallikrein and kallikrein-like serine proteases. The four enzymes share between 74% and 86% amino acid sequence identity and are identical in length with the exception of single two amino acid deletions in the S2 and S3 enzymes. The PS enzyme appears to be a true tissue kallikrein. The S1 enzyme shares 86% amino acid sequence homology with the PS enzyme and retains key amino acid residues thought to be primary determinants of kallikrein cleavage specificity. The S2 enzyme is rat submaxillary tonin. The amino acid sequence of the S3 enzyme is identical with tonin at 84% of its amino acid positions and retains the same amino acid substitutions at positions likely to determine substrate cleavage preferences.

Full Text

Duke Authors

Cited Authors

  • Ashley, PL; MacDonald, RJ

Published Date

  • August 13, 1985

Published In

Volume / Issue

  • 24 / 17

Start / End Page

  • 4512 - 4520

PubMed ID

  • 2998455

Pubmed Central ID

  • 2998455

International Standard Serial Number (ISSN)

  • 0006-2960

Digital Object Identifier (DOI)

  • 10.1021/bi00338a005

Language

  • eng

Conference Location

  • United States