Genome-wide analysis of central corneal thickness in primary open-angle glaucoma cases in the NEIGHBOR and GLAUGEN consortia.

Journal Article (Journal Article;Multicenter Study)

PURPOSE: To investigate the effects of central corneal thickness (CCT)-associated variants on primary open-angle glaucoma (POAG) risk using single nucleotide polymorphisms (SNP) data from the Glaucoma Genes and Environment (GLAUGEN) and National Eye Institute (NEI) Glaucoma Human Genetics Collaboration (NEIGHBOR) consortia. METHODS: A replication analysis of previously reported CCT SNPs was performed in a CCT dataset (n = 1117) and these SNPs were then tested for association with POAG using a larger POAG dataset (n = 6470). Then a CCT genome-wide association study (GWAS) was performed. Top SNPs from this analysis were selected and tested for association with POAG. cDNA libraries from fetal and adult brain and ocular tissue samples were generated and used for candidate gene expression analysis. RESULTS: Association with one of 20 previously published CCT SNPs was replicated: rs12447690, near the ZNF469 gene (P = 0.001; β = -5.08 μm/allele). None of these SNPs were significantly associated with POAG. In the CCT GWAS, no SNPs reached genome-wide significance. After testing 50 candidate SNPs for association with POAG, one SNP was identified, rs7481514 within the neurotrimin (NTM) gene, that was significantly associated with POAG in a low-tension subset (P = 0.00099; Odds Ratio [OR] = 1.28). Additionally, SNPs in the CNTNAP4 gene showed suggestive association with POAG (top SNP = rs1428758; P = 0.018; OR = 0.84). NTM and CNTNAP4 were shown to be expressed in ocular tissues. CONCLUSIONS: The results suggest previously reported CCT loci are not significantly associated with POAG susceptibility. By performing a quantitative analysis of CCT and a subsequent analysis of POAG, SNPs in two cell adhesion molecules, NTM and CNTNAP4, were identified and may increase POAG susceptibility in a subset of cases.

Full Text

Duke Authors

Cited Authors

  • Ulmer, M; Li, J; Yaspan, BL; Ozel, AB; Richards, JE; Moroi, SE; Hawthorne, F; Budenz, DL; Friedman, DS; Gaasterland, D; Haines, J; Kang, JH; Lee, R; Lichter, P; Liu, Y; Pasquale, LR; Pericak-Vance, M; Realini, A; Schuman, JS; Singh, K; Vollrath, D; Weinreb, R; Wollstein, G; Zack, DJ; Zhang, K; Young, T; Allingham, RR; Wiggs, JL; Ashley-Koch, A; Hauser, MA

Published Date

  • July 3, 2012

Published In

Volume / Issue

  • 53 / 8

Start / End Page

  • 4468 - 4474

PubMed ID

  • 22661486

Pubmed Central ID

  • PMC3394688

Electronic International Standard Serial Number (EISSN)

  • 1552-5783

Digital Object Identifier (DOI)

  • 10.1167/iovs.12-9784


  • eng

Conference Location

  • United States