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Enhancing melanoma treatment with resveratrol.

Publication ,  Journal Article
Osmond, GW; Augustine, CK; Zipfel, PA; Padussis, J; Tyler, DS
Published in: The Journal of surgical research
January 2012

Resveratrol (RESV) is a naturally occurring compound that possesses anti-cancer capabilities. The goal of this study was to evaluate the potential of RESV as an adjunct to chemotherapy in melanoma treatment.The in vitro and in vivo cytotoxic activity of RESV with or without chemotherapy was tested using cellular assays and a xenograft model. Two Duke melanoma cell lines (DM738, DM443) were used for both in vivo and in vitro experiments, and two nonmalignant human fibroblast lines (NHDF, HS68) were used for in vitro cellular assays. Xenografts were randomized to treatment arms and tumors measured to evaluate response. Results were analyzed using a Student's t-test and ANOVA. Western blots were performed on in vivo tissue.In vitro RESV significantly decreased melanoma cell viability in all lines tested (all P < 0.0001). Treatment of fibroblast cell lines revealed that RESV selectively spared NHDF and HS68 cells compared with its cytotoxic effects on melanoma cells (P < 0.0001). Treatment of malignant cells with 50 μM RESV and temozolomide (TMZ) for 72 h significantly enhanced cytotoxicity compared with treatment with TMZ alone (P < 0.0001). In vivo, however, there was no significant difference between any treatment arms (P = 0.65).RESV shows promise as a novel therapeutic in the management of melanoma for its selective anti-tumor activity in vitro. Translating in vitro results to in vivo models has proven difficult. Barriers thought to prevent such translation are identified, and a rationale for overcoming them is discussed.

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Published In

The Journal of surgical research

DOI

EISSN

1095-8673

ISSN

0022-4804

Publication Date

January 2012

Volume

172

Issue

1

Start / End Page

109 / 115

Related Subject Headings

  • Xenograft Model Antitumor Assays
  • Treatment Outcome
  • Temozolomide
  • Surgery
  • Stilbenes
  • Skin Neoplasms
  • Resveratrol
  • Mice, SCID
  • Mice, Nude
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
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Osmond, G. W., Augustine, C. K., Zipfel, P. A., Padussis, J., & Tyler, D. S. (2012). Enhancing melanoma treatment with resveratrol. The Journal of Surgical Research, 172(1), 109–115. https://doi.org/10.1016/j.jss.2010.07.033
Osmond, Gregory W., Christina K. Augustine, Patricia A. Zipfel, James Padussis, and Douglas S. Tyler. “Enhancing melanoma treatment with resveratrol.The Journal of Surgical Research 172, no. 1 (January 2012): 109–15. https://doi.org/10.1016/j.jss.2010.07.033.
Osmond GW, Augustine CK, Zipfel PA, Padussis J, Tyler DS. Enhancing melanoma treatment with resveratrol. The Journal of surgical research. 2012 Jan;172(1):109–15.
Osmond, Gregory W., et al. “Enhancing melanoma treatment with resveratrol.The Journal of Surgical Research, vol. 172, no. 1, Jan. 2012, pp. 109–15. Epmc, doi:10.1016/j.jss.2010.07.033.
Osmond GW, Augustine CK, Zipfel PA, Padussis J, Tyler DS. Enhancing melanoma treatment with resveratrol. The Journal of surgical research. 2012 Jan;172(1):109–115.
Journal cover image

Published In

The Journal of surgical research

DOI

EISSN

1095-8673

ISSN

0022-4804

Publication Date

January 2012

Volume

172

Issue

1

Start / End Page

109 / 115

Related Subject Headings

  • Xenograft Model Antitumor Assays
  • Treatment Outcome
  • Temozolomide
  • Surgery
  • Stilbenes
  • Skin Neoplasms
  • Resveratrol
  • Mice, SCID
  • Mice, Nude
  • Mice