Plasma membrane polarization during mating in yeast cells.

Journal Article

The yeast mating cell provides a simple paradigm for analyzing mechanisms underlying the generation of surface polarity. Endocytic recycling and slow diffusion on the plasma membrane were shown to facilitate polarized surface distribution of Snc1p (Valdez-Taubas, J., and H.R. Pelham. 2003. Curr. Biol. 13:1636-1640). Here, we found that polarization of Fus1p, a raft-associated type I transmembrane protein involved in cell fusion, does not depend on endocytosis. Instead, Fus1p localization to the tip of the mating projection was determined by its cytosolic domain, which binds to peripheral proteins involved in mating tip polarization. Furthermore, we provide evidence that the lipid bilayer at the mating projection is more condensed than the plasma membrane enclosing the cell body, and that sphingolipids are required for this lipid organization.

Full Text

Duke Authors

Cited Authors

  • Proszynski, TJ; Klemm, R; Bagnat, M; Gaus, K; Simons, K

Published Date

  • June 19, 2006

Published In

Volume / Issue

  • 173 / 6

Start / End Page

  • 861 - 866

PubMed ID

  • 16769822

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.200602007

Language

  • eng

Conference Location

  • United States