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A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast.

Publication ,  Journal Article
Proszynski, TJ; Klemm, RW; Gravert, M; Hsu, PP; Gloor, Y; Wagner, J; Kozak, K; Grabner, H; Walzer, K; Bagnat, M; Simons, K; Walch-Solimena, C
Published in: Proc Natl Acad Sci U S A
December 13, 2005

Recently synthesized proteins are sorted at the trans-Golgi network into specialized routes for exocytosis. Surprisingly little is known about the underlying molecular machinery. Here, we present a visual screen to search for proteins involved in cargo sorting and vesicle formation. We expressed a GFP-tagged plasma membrane protein in the yeast deletion library and identified mutants with altered marker localization. This screen revealed a requirement of several enzymes regulating the synthesis of sphingolipids and ergosterol in the correct and efficient delivery of the marker protein to the cell surface. Additionally, we identified mutants regulating the actin cytoskeleton (Rvs161p and Vrp1p), known membrane traffic regulators (Kes1p and Chs5p), and several unknown genes. This visual screening method can now be used for different cargo proteins to search in a genome-wide fashion for machinery involved in post-Golgi sorting.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 13, 2005

Volume

102

Issue

50

Start / End Page

17981 / 17986

Location

United States

Related Subject Headings

  • trans-Golgi Network
  • Vesicular Transport Proteins
  • Transport Vesicles
  • Sphingolipids
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Phenotype
  • Mutant Chimeric Proteins
  • Membrane Proteins
  • Membrane Glycoproteins
 

Citation

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Proszynski, T. J., Klemm, R. W., Gravert, M., Hsu, P. P., Gloor, Y., Wagner, J., … Walch-Solimena, C. (2005). A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast. Proc Natl Acad Sci U S A, 102(50), 17981–17986. https://doi.org/10.1073/pnas.0509107102
Proszynski, Tomasz J., Robin W. Klemm, Maike Gravert, Peggy P. Hsu, Yvonne Gloor, Jan Wagner, Karol Kozak, et al. “A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast.Proc Natl Acad Sci U S A 102, no. 50 (December 13, 2005): 17981–86. https://doi.org/10.1073/pnas.0509107102.
Proszynski TJ, Klemm RW, Gravert M, Hsu PP, Gloor Y, Wagner J, et al. A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast. Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):17981–6.
Proszynski, Tomasz J., et al. “A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast.Proc Natl Acad Sci U S A, vol. 102, no. 50, Dec. 2005, pp. 17981–86. Pubmed, doi:10.1073/pnas.0509107102.
Proszynski TJ, Klemm RW, Gravert M, Hsu PP, Gloor Y, Wagner J, Kozak K, Grabner H, Walzer K, Bagnat M, Simons K, Walch-Solimena C. A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast. Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):17981–17986.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 13, 2005

Volume

102

Issue

50

Start / End Page

17981 / 17986

Location

United States

Related Subject Headings

  • trans-Golgi Network
  • Vesicular Transport Proteins
  • Transport Vesicles
  • Sphingolipids
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Phenotype
  • Mutant Chimeric Proteins
  • Membrane Proteins
  • Membrane Glycoproteins