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Glycophase glass revisited: protein adsorption and cell growth on glass surfaces bearing immobilized glycerol monosaccharides.

Publication ,  Journal Article
Bain, JR; Hoffman, AS
Published in: Biomaterials
August 2002

Gamma-Glycerylpropylsilyl or "glycophase" glass has been promoted as a non-fouling surface, resistant to protein adsorption and cell attachment, on which one can immobilize oligopeptide ligands, and thus create cell-type-specific culture surfaces. The present study confirmed that the glycerol-rich glycophase surface is a useful support for peptide immobilization. But glycophase glass was observed to adsorb more albumin than glass. At pH 7.4, desorption studies revealed that albumin bound more tightly to glycophase glass than to glass. Moreover, the growth rates, morphologies, and functions of transgenic betaG I/17 insulinoma cell cultures were equivalent on the two surfaces. Glycophase glass is neither protein- nor cell-repellant.

Duke Scholars

Published In

Biomaterials

DOI

ISSN

0142-9612

Publication Date

August 2002

Volume

23

Issue

16

Start / End Page

3347 / 3357

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Silicates
  • Serum Albumin, Bovine
  • Pancreatic Neoplasms
  • Oligopeptides
  • Monosaccharides
  • Molecular Structure
  • Insulinoma
  • Insulin Secretion
  • Insulin
 

Citation

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Bain, J. R., & Hoffman, A. S. (2002). Glycophase glass revisited: protein adsorption and cell growth on glass surfaces bearing immobilized glycerol monosaccharides. Biomaterials, 23(16), 3347–3357. https://doi.org/10.1016/s0142-9612(02)00035-2
Bain, James R., and Allan S. Hoffman. “Glycophase glass revisited: protein adsorption and cell growth on glass surfaces bearing immobilized glycerol monosaccharides.Biomaterials 23, no. 16 (August 2002): 3347–57. https://doi.org/10.1016/s0142-9612(02)00035-2.
Bain, James R., and Allan S. Hoffman. “Glycophase glass revisited: protein adsorption and cell growth on glass surfaces bearing immobilized glycerol monosaccharides.Biomaterials, vol. 23, no. 16, Aug. 2002, pp. 3347–57. Pubmed, doi:10.1016/s0142-9612(02)00035-2.
Journal cover image

Published In

Biomaterials

DOI

ISSN

0142-9612

Publication Date

August 2002

Volume

23

Issue

16

Start / End Page

3347 / 3357

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Silicates
  • Serum Albumin, Bovine
  • Pancreatic Neoplasms
  • Oligopeptides
  • Monosaccharides
  • Molecular Structure
  • Insulinoma
  • Insulin Secretion
  • Insulin