Glycophase glass revisited: protein adsorption and cell growth on glass surfaces bearing immobilized glycerol monosaccharides.

Published

Journal Article

Gamma-Glycerylpropylsilyl or "glycophase" glass has been promoted as a non-fouling surface, resistant to protein adsorption and cell attachment, on which one can immobilize oligopeptide ligands, and thus create cell-type-specific culture surfaces. The present study confirmed that the glycerol-rich glycophase surface is a useful support for peptide immobilization. But glycophase glass was observed to adsorb more albumin than glass. At pH 7.4, desorption studies revealed that albumin bound more tightly to glycophase glass than to glass. Moreover, the growth rates, morphologies, and functions of transgenic betaG I/17 insulinoma cell cultures were equivalent on the two surfaces. Glycophase glass is neither protein- nor cell-repellant.

Full Text

Duke Authors

Cited Authors

  • Bain, JR; Hoffman, AS

Published Date

  • August 2002

Published In

Volume / Issue

  • 23 / 16

Start / End Page

  • 3347 - 3357

PubMed ID

  • 12099277

Pubmed Central ID

  • 12099277

International Standard Serial Number (ISSN)

  • 0142-9612

Language

  • eng

Conference Location

  • Netherlands