Inhibition of Epstein-Barr virus (EBV)-specific CD8+
cytotoxic T lymphocyte (CTL) activity by soluble HLA class I in vitro
In the present study, the effects of soluble HLA (sHLA) class I molecules against EBV-specific CTL were examined. Two different sources of sHLA class I, either bioengineered spliced form of HLA-B7 (sB7) or natural production from EBV-transformed B cells (natural sHLA), were added during the induction of CTL or incubated with MHC-restricted CD8+ CTL, which were selected by immunobeads just before testing for their cytotoxic activity. Both sB7 and natural sHLA class I blocked the generation of CD8+ CTL and also inhibited the cytotoxic activity of established CTL in a dose-dependent manner. In both ways, natural sHLA class I was effective in 10-fold lower concentrations compared with sB7. The inhibitory effect did not require a sharing of the HLA allotypes between sHLA and the CTL. CTL, after being treated with sHLA, underwent apoptosis, which was considered here as the main mechanism.
Gansuvd, B; Hagihara, M; Munkhbat, B; Kanai, N; Morita, N; Munkhtuvshin, N; Chargui, J; Kato, S; Hotta, T; Tsuji, K
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