Beta-arrestin 2 regulates zebrafish development through the hedgehog signaling pathway.

Published

Journal Article

beta-arrestins are multifunctional proteins that act as scaffolds and transducers of intracellular signals from heptahelical transmembrane-spanning receptors (7TMR). Hedgehog (Hh) signaling, which uses the putative 7TMR, Smoothened, is established as a fundamental pathway in development, and unregulated Hh signaling is associated with certain malignancies. Here, we show that the functional knockdown of beta-arrestin 2 in zebrafish embryos recapitulates the many phenotypes of Hh pathway mutants. Expression of wild-type beta-arrestin 2, or constitutive activation of the Hh pathway downstream of Smoothened, rescues the phenotypes caused by beta-arrestin 2 deficiency. These results suggest that a functional interaction between beta-arrestin 2 and Smoothened may be critical to regulate Hh signaling in zebrafish development.

Full Text

Duke Authors

Cited Authors

  • Wilbanks, AM; Fralish, GB; Kirby, ML; Barak, LS; Li, Y-X; Caron, MG

Published Date

  • December 2004

Published In

Volume / Issue

  • 306 / 5705

Start / End Page

  • 2264 - 2267

PubMed ID

  • 15618520

Pubmed Central ID

  • 15618520

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.1104193

Language

  • eng