Detection of minor drug-resistant populations by parallel allele-specific sequencing.
Journal Article (Journal Article)
We developed a highly sensitive parallel allele-specific sequencing (PASS) assay to simultaneously analyze a large number of viral genomes and detect minor drug-resistant populations at approximately 0.1-0.01% levels. Using this assay on samples from individuals infected with human immunodeficiency viruses (HIV), we successfully detected and quantified minor populations of drug-resistant viruses and performed linkage analysis of multiple-drug resistance mutations. This assay may serve as a useful tool to study drug resistance in HIV and other infectious agents.
- Cai, F; Chen, H; Hicks, CB; Bartlett, JA; Zhu, J; Gao, F
- February 2007
Volume / Issue
- 4 / 2
Start / End Page
- 123 - 125
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)
- United States