Detection of minor drug-resistant populations by parallel allele-specific sequencing.

Journal Article (Journal Article)

We developed a highly sensitive parallel allele-specific sequencing (PASS) assay to simultaneously analyze a large number of viral genomes and detect minor drug-resistant populations at approximately 0.1-0.01% levels. Using this assay on samples from individuals infected with human immunodeficiency viruses (HIV), we successfully detected and quantified minor populations of drug-resistant viruses and performed linkage analysis of multiple-drug resistance mutations. This assay may serve as a useful tool to study drug resistance in HIV and other infectious agents.

Full Text

Duke Authors

Cited Authors

  • Cai, F; Chen, H; Hicks, CB; Bartlett, JA; Zhu, J; Gao, F

Published Date

  • February 2007

Published In

Volume / Issue

  • 4 / 2

Start / End Page

  • 123 - 125

PubMed ID

  • 17206150

International Standard Serial Number (ISSN)

  • 1548-7091

Digital Object Identifier (DOI)

  • 10.1038/nmeth995


  • eng

Conference Location

  • United States