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Platelet and fibrin modification by radiographic contrast media.

Publication ,  Journal Article
Gabriel, DA; Jones, MR; Reece, NS; Boothroyd, E; Bashore, T
Published in: Circ Res
March 1991

The effect of the radiographic contrast agents, iopamidol and diatrizoate, on fibrin assembly and structure as well as platelet surface charge was studied. Increasing the iopamidol concentration from 0 to 4.5 mM prolongs the fibrin gelation time from 20 to 105 seconds (an anticoagulant effect) and reduces the fibrin fiber mass/length ratio from 3.2 x 10(12) to 0.5 x 10(12) Da/cm (i.e., produces very thin fibrin fibers). Ultraviolet difference spectroscopy of fibrinogen showed both a 15-nm shift in the ultraviolet difference maximum for iopamidol (suggesting binding) and a perturbation of the aromatic amino acid side chain region for fibrinogen (suggesting a conformational change in fibrinogen) as the concentration of iopamidol was increased from 0 to 9 mg/ml. Binding of iopamidol to fibrinogen was also shown by affinity chromatography using a Sepharose-fibrinogen column. Electrophoretic quasi elastic light scattering was used to show platelet interaction with iopamidol as reflected in a reduction in the platelet electrophoretic mobility from 2.0 to 0.5 (microns-cm)/(V-sec) as the concentration of iopamidol was increased from 0 to 4.5 mM. In addition, the ionic radiopaque contrast agent, Renografin, was also studied and found to inhibit fibrin monomer assembly. Although iopamidol is not shown to be thrombogenic, iopamidol does appear to reduce platelet surface charge, bind fibrinogen, and modify fibrin clot structure.

Duke Scholars

Published In

Circ Res

DOI

ISSN

0009-7330

Publication Date

March 1991

Volume

68

Issue

3

Start / End Page

881 / 887

Location

United States

Related Subject Headings

  • Scattering, Radiation
  • Light
  • Iopamidol
  • Humans
  • Fibrin
  • Electrophoresis
  • Diatrizoate
  • Contrast Media
  • Cardiovascular System & Hematology
  • Blood Platelets
 

Citation

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MLA
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Gabriel, D. A., Jones, M. R., Reece, N. S., Boothroyd, E., & Bashore, T. (1991). Platelet and fibrin modification by radiographic contrast media. Circ Res, 68(3), 881–887. https://doi.org/10.1161/01.res.68.3.881
Gabriel, D. A., M. R. Jones, N. S. Reece, E. Boothroyd, and T. Bashore. “Platelet and fibrin modification by radiographic contrast media.Circ Res 68, no. 3 (March 1991): 881–87. https://doi.org/10.1161/01.res.68.3.881.
Gabriel DA, Jones MR, Reece NS, Boothroyd E, Bashore T. Platelet and fibrin modification by radiographic contrast media. Circ Res. 1991 Mar;68(3):881–7.
Gabriel, D. A., et al. “Platelet and fibrin modification by radiographic contrast media.Circ Res, vol. 68, no. 3, Mar. 1991, pp. 881–87. Pubmed, doi:10.1161/01.res.68.3.881.
Gabriel DA, Jones MR, Reece NS, Boothroyd E, Bashore T. Platelet and fibrin modification by radiographic contrast media. Circ Res. 1991 Mar;68(3):881–887.

Published In

Circ Res

DOI

ISSN

0009-7330

Publication Date

March 1991

Volume

68

Issue

3

Start / End Page

881 / 887

Location

United States

Related Subject Headings

  • Scattering, Radiation
  • Light
  • Iopamidol
  • Humans
  • Fibrin
  • Electrophoresis
  • Diatrizoate
  • Contrast Media
  • Cardiovascular System & Hematology
  • Blood Platelets