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Mn porphyrins as novel molecular magnetic resonance imaging contrast agents.

Publication ,  Journal Article
Mouraviev, V; Venkatraman, TN; Tovmasyan, A; Kimura, M; Tsivian, M; Mouravieva, V; Polascik, TJ; Wang, H; Amrhein, TJ; Batinic-Haberle, I; Lascola, C
Published in: J Endourol
November 2012

BACKGROUND AND PURPOSE: In this study, we investigated the potential of a new class of therapeutic Mn porphyrins as molecular MRI probes for prostate cancer imaging. Two compounds of different bioavailibility were investigated: Mn(III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin (MnTE-2-PyP(5+)) and Mn(III) meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin (MnTnHex-2-PyP(5+)). These compounds have previously been shown to have adjunctive antineoplastic activity through their actions as powerful superoxide dismutase mimics, peroxynitrite scavengers, and modulators of cellular redox-based signaling pathways. Strong paramagnetic MRI contrast properties and affinity for cancer cells suggest their potential application as novel diagnostic imaging agents. MATERIALS AND METHODS: MRI experiments were performed at 7.0T on a Bruker Biospec horizontal bore scanner. All in-vivo experiments were performed on 12 C57 black mice implanted with RM-9 prostate cancer cells on the hind limb. Two mg/kg of MnTnHex-2-PyP(5+) (n=6) and 8 mg/kg MnTE-2-PyP(5+) (n=6) were administered intraperitoneally 90 minutes before imaging. All the images were collected using a volume coil and processed using Paravision 4.0. RESULTS: Phantom studies reveal remarkably high T1 relaxivity changes for both metalloporphyrins, which are twofold to threefold higher than commercially available gadolinium chelates. Observable detection limits using conventional T1-weighted MRI are in the low micromolar range for both compounds. In vivo, MR relaxation changes in prostate tumor xenografts were readily observed after a single injection of either MnTE-2-PyP(5+)or MnTnHex-2-PyP(5+), with tumor contrast to background ratio greatest after MnTE-2-PyP(5+) administration. CONCLUSION: After a single dose of MnTE-2-PyP(5+), contrast changes in prostate tumors are up to sixfold greater than in surrounding, noncancerous tissues, suggesting the potential use of this metalloporphyrin as a novel diagnostic probe for detecting prostate malignancy using MRI.

Duke Scholars

Published In

J Endourol

DOI

EISSN

1557-900X

Publication Date

November 2012

Volume

26

Issue

11

Start / End Page

1420 / 1424

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Prostatic Neoplasms
  • Phantoms, Imaging
  • Mice, Inbred C57BL
  • Mice
  • Metalloporphyrins
  • Manganese
  • Male
  • Magnetic Resonance Imaging
  • Humans
 

Citation

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Mouraviev, V., Venkatraman, T. N., Tovmasyan, A., Kimura, M., Tsivian, M., Mouravieva, V., … Lascola, C. (2012). Mn porphyrins as novel molecular magnetic resonance imaging contrast agents. J Endourol, 26(11), 1420–1424. https://doi.org/10.1089/end.2012.0171
Mouraviev, Vladimir, Talaignair N. Venkatraman, Artak Tovmasyan, Masaki Kimura, Matvey Tsivian, Vladimira Mouravieva, Tom J. Polascik, et al. “Mn porphyrins as novel molecular magnetic resonance imaging contrast agents.J Endourol 26, no. 11 (November 2012): 1420–24. https://doi.org/10.1089/end.2012.0171.
Mouraviev V, Venkatraman TN, Tovmasyan A, Kimura M, Tsivian M, Mouravieva V, et al. Mn porphyrins as novel molecular magnetic resonance imaging contrast agents. J Endourol. 2012 Nov;26(11):1420–4.
Mouraviev, Vladimir, et al. “Mn porphyrins as novel molecular magnetic resonance imaging contrast agents.J Endourol, vol. 26, no. 11, Nov. 2012, pp. 1420–24. Pubmed, doi:10.1089/end.2012.0171.
Mouraviev V, Venkatraman TN, Tovmasyan A, Kimura M, Tsivian M, Mouravieva V, Polascik TJ, Wang H, Amrhein TJ, Batinic-Haberle I, Lascola C. Mn porphyrins as novel molecular magnetic resonance imaging contrast agents. J Endourol. 2012 Nov;26(11):1420–1424.
Journal cover image

Published In

J Endourol

DOI

EISSN

1557-900X

Publication Date

November 2012

Volume

26

Issue

11

Start / End Page

1420 / 1424

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Prostatic Neoplasms
  • Phantoms, Imaging
  • Mice, Inbred C57BL
  • Mice
  • Metalloporphyrins
  • Manganese
  • Male
  • Magnetic Resonance Imaging
  • Humans