Amelioration of renal ischemia-reperfusion injury with a novel protective cocktail.
Journal Article (Journal Article)
PURPOSE: Extended warm ischemia during partial nephrectomy can lead to considerable renal injury. Using a rat model of renal ischemia we examined the ability of a unique renoprotective cocktail to ameliorate warm ischemia-reperfusion injury. MATERIALS AND METHODS: A warm renal ischemia model was developed using 60 Sprague-Dawley® rats. The left renal artery was clamped for 40 minutes, followed by 48 hours of reperfusion. A renoprotective cocktail of a mixture of specific growth factors, mitochondria protecting biochemicals and Manganese-Porphyrin (MnTnHex-2-PyP(5+)) was given intramuscularly at -24, 0 and 24 hours after surgery. At 48 hours the 2 kidneys were harvested and examined with hematoxylin and eosin, and periodic acid-Schiff stains. Protein and gene expression were also analyzed to determine ischemia markers and the antioxidant response. RESULTS: Compared to ischemic controls, kidneys treated with the renoprotective cocktail showed significant reversal of morphological changes and a significant decrease in the specific ischemic markers lipocalin-2, mucin-1 and galectin-3. Quantitative reverse transcriptase-polymerase chain reaction revealed up-regulation of several antioxidant genes in treated animals. CONCLUSIONS: According to histopathological and several molecular measures our unique renoprotective cocktail mitigated ischemia-reperfusion injury.
Full Text
Duke Authors
Cited Authors
- Dorai, T; Fishman, AI; Ding, C; Batinic-Haberle, I; Goldfarb, DS; Grasso, M
Published Date
- December 2011
Published In
Volume / Issue
- 186 / 6
Start / End Page
- 2448 - 2454
PubMed ID
- 22019164
Electronic International Standard Serial Number (EISSN)
- 1527-3792
Digital Object Identifier (DOI)
- 10.1016/j.juro.2011.08.010
Language
- eng
Conference Location
- United States