Induction of oxidative cell damage by photo-treatment with zinc meta N-methylpyridylporphyrin.

Published

Journal Article

We have previously reported that isomeric Zn(II) N-methylpyridylporphyrins (ZnTM-2(3,4)-PyP4 + ) can act as photosensitizers with efficacy comparable to that of hematoporphyrin derivative (HpD) in preventing cell proliferation and causing cell death in vitro. To better understand the biochemical basis of this activity, the effects of photo-activated ZnTM-3-PyP4 + on GSH/GSSG ratio, lipid peroxidation, membrane permeability, oxidative DNA damage, and the activities of SOD, catalase, glutathione reductase, and glutathione peroxidase were evaluated. Light exposure of ZnTM-3-PyP4 + -treated colon adenocarcinoma cells caused a wide spectrum of oxidative damage including depletion of GSH, inactivation of glutathione reductase and glutathione peroxidase, oxidative DNA damage and peroxidation of membrane lipids. Cell staining with Hoechst-33342 showed morphological changes consistent with both necrotic and apoptotic death sequences, depending upon the presence of oxygen.

Full Text

Duke Authors

Cited Authors

  • Al-Mutairi, DA; Craik, JD; Batinic-Haberle, I; Benov, LT

Published Date

  • January 1, 2007

Published In

Volume / Issue

  • 41 / 1

Start / End Page

  • 89 - 96

PubMed ID

  • 17164182

Pubmed Central ID

  • 17164182

International Standard Serial Number (ISSN)

  • 1071-5762

Digital Object Identifier (DOI)

  • 10.1080/10715760600952869

Language

  • eng

Conference Location

  • England