Essential role of an active-site guanine in glmS ribozyme catalysis.

Journal Article

The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction.

Full Text

Duke Authors

Cited Authors

  • Klein, DJ; Been, MD; Ferré-D'Amaré, AR

Published Date

  • December 5, 2007

Published In

Volume / Issue

  • 129 / 48

Start / End Page

  • 14858 - 14859

PubMed ID

  • 17990888

Electronic International Standard Serial Number (EISSN)

  • 1520-5126

Digital Object Identifier (DOI)

  • 10.1021/ja0768441

Language

  • eng

Conference Location

  • United States