Essential role of an active-site guanine in glmS ribozyme catalysis.
Publication
, Journal Article
Klein, DJ; Been, MD; Ferré-D'Amaré, AR
Published in: J Am Chem Soc
December 5, 2007
The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction.
Duke Scholars
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Published In
J Am Chem Soc
DOI
EISSN
1520-5126
Publication Date
December 5, 2007
Volume
129
Issue
48
Start / End Page
14858 / 14859
Location
United States
Related Subject Headings
- Thermoanaerobacter
- RNA, Catalytic
- Mutation
- Molecular Structure
- Models, Molecular
- Guanine
- General Chemistry
- Crystallography, X-Ray
- Catalysis
- Binding Sites
Citation
APA
Chicago
ICMJE
MLA
NLM
Klein, D. J., Been, M. D., & Ferré-D’Amaré, A. R. (2007). Essential role of an active-site guanine in glmS ribozyme catalysis. J Am Chem Soc, 129(48), 14858–14859. https://doi.org/10.1021/ja0768441
Klein, Daniel J., Michael D. Been, and Adrian R. Ferré-D’Amaré. “Essential role of an active-site guanine in glmS ribozyme catalysis.” J Am Chem Soc 129, no. 48 (December 5, 2007): 14858–59. https://doi.org/10.1021/ja0768441.
Klein DJ, Been MD, Ferré-D’Amaré AR. Essential role of an active-site guanine in glmS ribozyme catalysis. J Am Chem Soc. 2007 Dec 5;129(48):14858–9.
Klein, Daniel J., et al. “Essential role of an active-site guanine in glmS ribozyme catalysis.” J Am Chem Soc, vol. 129, no. 48, Dec. 2007, pp. 14858–59. Pubmed, doi:10.1021/ja0768441.
Klein DJ, Been MD, Ferré-D’Amaré AR. Essential role of an active-site guanine in glmS ribozyme catalysis. J Am Chem Soc. 2007 Dec 5;129(48):14858–14859.
Published In
J Am Chem Soc
DOI
EISSN
1520-5126
Publication Date
December 5, 2007
Volume
129
Issue
48
Start / End Page
14858 / 14859
Location
United States
Related Subject Headings
- Thermoanaerobacter
- RNA, Catalytic
- Mutation
- Molecular Structure
- Models, Molecular
- Guanine
- General Chemistry
- Crystallography, X-Ray
- Catalysis
- Binding Sites