RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods

Published

Journal Article

RNA enzymes or ribozymes can act as endoribonucleases, catalyzing the cleavage of RNA molecules with a sequence specificity of cleavage greater than that of known ribonucleases and approaching that of the DNA restriction endonucleases, thus serving as RNA sequence specific endoribonucleases. An example is a shortened form of the self-splicing ribosomal RNA intervening sequence of Tetrahymena (L- 19 IVS RNA). Site-specific mutagenesis of the enzyme active site of the L-19 IVS RNA alters the substrate sequence specificity in a predictable manner, allowing a set of sequence-specific endoribonucleases to be synthesized. Varying conditions allow the ribozyme to act as a polymerase (nucleotidyltransferase), a dephosphorylase (acid phosphatase or phosphotransferase) or a sequence-specific endoribonuclease.

Full Text

Duke Authors

Cited Authors

  • Cech, TR; Zaug, AJ; Been, M

Published Date

  • January 1, 1997

Published In

Volume / Issue

  • 15 / 3-4

Start / End Page

  • 666 -

International Standard Serial Number (ISSN)

  • 0734-9750

Digital Object Identifier (DOI)

  • 10.1016/S0734-9750(97)87520-9

Citation Source

  • Scopus