Generation and characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P protein.
A circular form of Bacillus subtilis ribonuclease P RNA (C-P RNA) was generated in vitro by splicing permuted intron-exon (PIE) sequences containing the P RNA sequence. Steady-state cleavage of pre-tRNA(Asp) catalyzed by circular P RNA is slightly faster than the linear form. Furthermore, steady-state turnover catalyzed by circular RNase P RNA is activated by the addition of the Bacillus subtilis protein component of RNase P, to a rate constant equal to the linear holoenzyme under identical conditions. Also, the circles are resistant to nuclease degradation, have less sequence heterogeneity, and may enhance the formation of a unique structure. Therefore, circular forms of RNase P RNA should prove useful for mutagenesis and structural studies.
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- Ribonuclease P
- RNA, Transfer, Asp
- RNA, Circular
- RNA, Catalytic
- RNA, Bacterial
- RNA Splicing
- RNA Precursors
- RNA
- Molecular Structure
- Kinetics
Citation
Published In
ISSN
Publication Date
Issue
Start / End Page
Location
Related Subject Headings
- Ribonuclease P
- RNA, Transfer, Asp
- RNA, Circular
- RNA, Catalytic
- RNA, Bacterial
- RNA Splicing
- RNA Precursors
- RNA
- Molecular Structure
- Kinetics