Generation and characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P protein.

Journal Article

A circular form of Bacillus subtilis ribonuclease P RNA (C-P RNA) was generated in vitro by splicing permuted intron-exon (PIE) sequences containing the P RNA sequence. Steady-state cleavage of pre-tRNA(Asp) catalyzed by circular P RNA is slightly faster than the linear form. Furthermore, steady-state turnover catalyzed by circular RNase P RNA is activated by the addition of the Bacillus subtilis protein component of RNase P, to a rate constant equal to the linear holoenzyme under identical conditions. Also, the circles are resistant to nuclease degradation, have less sequence heterogeneity, and may enhance the formation of a unique structure. Therefore, circular forms of RNase P RNA should prove useful for mutagenesis and structural studies.

Full Text

Duke Authors

Cited Authors

  • Puttaraju, M; Beebe, JA; Niranjanakumari, S; Been, MD; Fierke, CA

Published Date

  • 1995

Published In

Start / End Page

  • 92 - 94

PubMed ID

  • 8643411

International Standard Serial Number (ISSN)

  • 0261-3166

Language

  • eng

Conference Location

  • England