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Generation and characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P protein.

Publication ,  Journal Article
Puttaraju, M; Beebe, JA; Niranjanakumari, S; Been, MD; Fierke, CA
Published in: Nucleic Acids Symp Ser
1995

A circular form of Bacillus subtilis ribonuclease P RNA (C-P RNA) was generated in vitro by splicing permuted intron-exon (PIE) sequences containing the P RNA sequence. Steady-state cleavage of pre-tRNA(Asp) catalyzed by circular P RNA is slightly faster than the linear form. Furthermore, steady-state turnover catalyzed by circular RNase P RNA is activated by the addition of the Bacillus subtilis protein component of RNase P, to a rate constant equal to the linear holoenzyme under identical conditions. Also, the circles are resistant to nuclease degradation, have less sequence heterogeneity, and may enhance the formation of a unique structure. Therefore, circular forms of RNase P RNA should prove useful for mutagenesis and structural studies.

Duke Scholars

Published In

Nucleic Acids Symp Ser

ISSN

0261-3166

Publication Date

1995

Issue

33

Start / End Page

92 / 94

Location

England

Related Subject Headings

  • Ribonuclease P
  • RNA, Transfer, Asp
  • RNA, Circular
  • RNA, Catalytic
  • RNA, Bacterial
  • RNA Splicing
  • RNA Precursors
  • RNA
  • Molecular Structure
  • Kinetics
 

Citation

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Puttaraju, M., Beebe, J. A., Niranjanakumari, S., Been, M. D., & Fierke, C. A. (1995). Generation and characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P protein. Nucleic Acids Symp Ser, (33), 92–94.
Puttaraju, M., J. A. Beebe, S. Niranjanakumari, M. D. Been, and C. A. Fierke. “Generation and characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P protein.Nucleic Acids Symp Ser, no. 33 (1995): 92–94.
Puttaraju M, Beebe JA, Niranjanakumari S, Been MD, Fierke CA. Generation and characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P protein. Nucleic Acids Symp Ser. 1995;(33):92–4.
Puttaraju, M., et al. “Generation and characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P protein.Nucleic Acids Symp Ser, no. 33, 1995, pp. 92–94.
Puttaraju M, Beebe JA, Niranjanakumari S, Been MD, Fierke CA. Generation and characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P protein. Nucleic Acids Symp Ser. 1995;(33):92–94.

Published In

Nucleic Acids Symp Ser

ISSN

0261-3166

Publication Date

1995

Issue

33

Start / End Page

92 / 94

Location

England

Related Subject Headings

  • Ribonuclease P
  • RNA, Transfer, Asp
  • RNA, Circular
  • RNA, Catalytic
  • RNA, Bacterial
  • RNA Splicing
  • RNA Precursors
  • RNA
  • Molecular Structure
  • Kinetics