Generation of nuclease resistant circular RNA decoys for HIV-Tat and HIV-Rev by autocatalytic splicing.
Publication
, Journal Article
Puttaraju, M; Been, MD
Published in: Nucleic Acids Symp Ser
1995
Circular exon sequences can be generated by splicing permuted intron-exon (PIE) sequences. The Anabaena pre-tRNA group I self-splicing PIE sequence was modified to generate circular forms of the HIV-TAR and the high affinity region of the HIV-RRE (RBE). RNA products containing TAR and the RBE were purified from splicing reactions and demonstrated to be circular. The circular form of these sequences was shown to be resistant to nuclease degradation in cellular extracts. Gel shift assays demonstrate that the circular form of the RBE is specifically bound by a Rev derived peptide. These data suggest that PIE-circularization of RNA may be an effective way to express small stable RNAs designed for therapeutics (eg-decoys).
Duke Scholars
Published In
Nucleic Acids Symp Ser
ISSN
0261-3166
Publication Date
1995
Issue
33
Start / End Page
152 / 155
Location
England
Related Subject Headings
- tat Gene Products, Human Immunodeficiency Virus
- rev Gene Products, Human Immunodeficiency Virus
- RNA, Viral
- RNA, Circular
- RNA Splicing
- RNA
- Nucleic Acid Conformation
- Molecular Sequence Data
- In Vitro Techniques
- Humans
Citation
APA
Chicago
ICMJE
MLA
NLM
Puttaraju, M., & Been, M. D. (1995). Generation of nuclease resistant circular RNA decoys for HIV-Tat and HIV-Rev by autocatalytic splicing. Nucleic Acids Symp Ser, (33), 152–155.
Puttaraju, M., and M. D. Been. “Generation of nuclease resistant circular RNA decoys for HIV-Tat and HIV-Rev by autocatalytic splicing.” Nucleic Acids Symp Ser, no. 33 (1995): 152–55.
Puttaraju M, Been MD. Generation of nuclease resistant circular RNA decoys for HIV-Tat and HIV-Rev by autocatalytic splicing. Nucleic Acids Symp Ser. 1995;(33):152–5.
Puttaraju, M., and M. D. Been. “Generation of nuclease resistant circular RNA decoys for HIV-Tat and HIV-Rev by autocatalytic splicing.” Nucleic Acids Symp Ser, no. 33, 1995, pp. 152–55.
Puttaraju M, Been MD. Generation of nuclease resistant circular RNA decoys for HIV-Tat and HIV-Rev by autocatalytic splicing. Nucleic Acids Symp Ser. 1995;(33):152–155.
Published In
Nucleic Acids Symp Ser
ISSN
0261-3166
Publication Date
1995
Issue
33
Start / End Page
152 / 155
Location
England
Related Subject Headings
- tat Gene Products, Human Immunodeficiency Virus
- rev Gene Products, Human Immunodeficiency Virus
- RNA, Viral
- RNA, Circular
- RNA Splicing
- RNA
- Nucleic Acid Conformation
- Molecular Sequence Data
- In Vitro Techniques
- Humans