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Cleavage of oligoribonucleotides by a ribozyme derived from the hepatitis delta virus RNA sequence.

Publication ,  Journal Article
Perrotta, AT; Been, MD
Published in: Biochemistry
January 14, 1992

A self-cleaving RNA sequence from hepatitis delta virus was modified to produce a ribozyme capable of catalyzing the cleavage of RNA in an intermolecular (trans) reaction. The delta-derived ribozyme cleaved substrate RNA at a specific site, and the sequence specificity could be altered with mutations in the region of the ribozyme proposed to base pair with the substrate. A substrate target size of approximately 8 nucleotides in length was identified. Octanucleotides containing a single ribonucleotide immediately 5' to the cleavage site were substrates for cleavage, and cleavage activity was significantly reduced only with a guanine base at that position. A deoxyribose 5' to the cleavage site blocked the reaction. These data are consistent with a proposed secondary structure for the self-cleaving form of the hepatitis delta virus ribozyme in which a duplex forms with sequences 3' to the cleavage site, and they support a proposed mechanism in which cleavage involves attack on the phosphorus at the cleavage site by the adjacent 2'-hydroxyl group.

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Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

January 14, 1992

Volume

31

Issue

1

Start / End Page

16 / 21

Location

United States

Related Subject Headings

  • Substrate Specificity
  • Sensitivity and Specificity
  • RNA, Viral
  • RNA, Catalytic
  • Oligoribonucleotides
  • Nucleosides
  • Nucleic Acid Conformation
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Hydrolysis
 

Citation

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Perrotta, A. T., & Been, M. D. (1992). Cleavage of oligoribonucleotides by a ribozyme derived from the hepatitis delta virus RNA sequence. Biochemistry, 31(1), 16–21. https://doi.org/10.1021/bi00116a004
Perrotta, A. T., and M. D. Been. “Cleavage of oligoribonucleotides by a ribozyme derived from the hepatitis delta virus RNA sequence.Biochemistry 31, no. 1 (January 14, 1992): 16–21. https://doi.org/10.1021/bi00116a004.
Perrotta, A. T., and M. D. Been. “Cleavage of oligoribonucleotides by a ribozyme derived from the hepatitis delta virus RNA sequence.Biochemistry, vol. 31, no. 1, Jan. 1992, pp. 16–21. Pubmed, doi:10.1021/bi00116a004.
Journal cover image

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

January 14, 1992

Volume

31

Issue

1

Start / End Page

16 / 21

Location

United States

Related Subject Headings

  • Substrate Specificity
  • Sensitivity and Specificity
  • RNA, Viral
  • RNA, Catalytic
  • Oligoribonucleotides
  • Nucleosides
  • Nucleic Acid Conformation
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Hydrolysis