A pseudoknot-like structure required for efficient self-cleavage of hepatitis delta virus RNA.

Published

Journal Article

Hepatitis delta virus genomic and antigenomic RNAs contain a self-cleavage site hypothesized to function in processing the viral RNA during replication. Self-cleavage requires only a divalent cation and is mediated at the genomic site by a sequence of less than 85 nucleotides. We propose that the genomic self-cleaving sequence element and a corresponding sequence from the anti-genomic RNA could generate related secondary structures. The region of the antigenomic sequence, predicted from the proposed structure, was synthesized and shown to be sufficient for self-cleavage. Evidence for two stems which form a tertiary interaction was obtained by site-specific mutagenesis of the antigenomic sequence. Efficient self-cleavage in 10 M formamide or 5 M urea, also a property of the genomic sequence, was dependent on base-pairing in both stems. But in the absence of denaturants, the stem distal to the site of cleavage was not required, suggesting that the tertiary interaction stabilizes the structure required for self-cleavage.

Full Text

Duke Authors

Cited Authors

  • Perrotta, AT; Been, MD

Published Date

  • April 4, 1991

Published In

Volume / Issue

  • 350 / 6317

Start / End Page

  • 434 - 436

PubMed ID

  • 2011192

Pubmed Central ID

  • 2011192

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/350434a0

Language

  • eng

Conference Location

  • England