Selection of circularization sites in a group I IVS RNA requires multiple alignments of an internal template-like sequence.


Journal Article

Circularization and reverse circularization of the Tetrahymena thermophila rRNA intervening sequence resemble the first and second steps in splicing, respectively. However, site-specific base substitutions show that different nucleotides are involved in selection of the 5' splice site and the circularization sites. Furthermore, a substitution at the major circularization site that prevents circularization can be suppressed by second substitutions at two different nucleotide positions. A model is proposed in which adjacent and overlapping sequences can function as a binding site, forming a short duplex with the sequence at the circularization site and thus directing circularization and reverse circularization. Because the 5' exon-binding site and three potential circularization binding sites fall within a contiguous eight nucleotide region, this sequence may translocate relative to the catalytic core of the ribozyme in a template-like manner.

Full Text

Duke Authors

Cited Authors

  • Been, MD; Cech, TR

Published Date

  • September 11, 1987

Published In

Volume / Issue

  • 50 / 6

Start / End Page

  • 951 - 961

PubMed ID

  • 2441876

Pubmed Central ID

  • 2441876

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(87)90522-8


  • eng

Conference Location

  • United States