The Tetrahymena ribozyme acts like an RNA restriction endonuclease.

Journal Article (Journal Article)

A shortened form of the Tetrahymena self-splicing ribosomal RNA intervening sequence acts as an endoribonuclease, catalysing the cleavage of large RNA molecules by a mechanism involving guanosine transfer. The sequence specificity approaches that of the DNA restriction endonucleases. Site-specific mutagenesis of the enzyme active site alters the substrate sequence specificity in a predictable manner, so that endoribonucleases can be synthesized to cut at a variety of tetranucleotide sequences.

Full Text

Duke Authors

Cited Authors

  • Zaug, AJ; Been, MD; Cech, TR

Published Date

  • December 4, 1986

Published In

Volume / Issue

  • 324 / 6096

Start / End Page

  • 429 - 433

PubMed ID

  • 3537808

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/324429a0


  • eng

Conference Location

  • England