Skip to main content
Journal cover image

One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity.

Publication ,  Journal Article
Been, MD; Cech, TR
Published in: Cell
October 24, 1986

The specificity of reactions catalyzed by the Tetrahymena pre-rRNA intervening sequence (IVS) was studied using site-specific mutagenesis. Two sequences required for 5' splice-site selection during self-splicing were defined. Single-base changes in either a 5' exon sequence or a 5' exon-binding site within the IVS disrupt their ability to pair and result in inefficient or inaccurate splicing. Combinations that restore complementarity suppress the effect of the single-base changes. Sequence alterations in the 5' exon-binding site also change the specificity of two other reactions: intermolecular exon ligation (trans-splicing) and the enzymatic nucleotidyltransferase activity of the IVS RNA. Thus the substrate specificity of an RNA enzyme can be changed in a manner predictable by the rules of Watson-Crick base-pairing.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

October 24, 1986

Volume

47

Issue

2

Start / End Page

207 / 216

Location

United States

Related Subject Headings

  • Tetrahymena
  • Templates, Genetic
  • Substrate Specificity
  • RNA, Ribosomal
  • RNA Splicing
  • Nucleotidyltransferases
  • Nucleic Acid Conformation
  • Mutation
  • Introns
  • Guanosine Triphosphate
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Been, M. D., & Cech, T. R. (1986). One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity. Cell, 47(2), 207–216. https://doi.org/10.1016/0092-8674(86)90443-5
Been, M. D., and T. R. Cech. “One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity.Cell 47, no. 2 (October 24, 1986): 207–16. https://doi.org/10.1016/0092-8674(86)90443-5.
Been, M. D., and T. R. Cech. “One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity.Cell, vol. 47, no. 2, Oct. 1986, pp. 207–16. Pubmed, doi:10.1016/0092-8674(86)90443-5.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

October 24, 1986

Volume

47

Issue

2

Start / End Page

207 / 216

Location

United States

Related Subject Headings

  • Tetrahymena
  • Templates, Genetic
  • Substrate Specificity
  • RNA, Ribosomal
  • RNA Splicing
  • Nucleotidyltransferases
  • Nucleic Acid Conformation
  • Mutation
  • Introns
  • Guanosine Triphosphate