Sites of circularization of the Tetrahymena rRNA IVS are determined by sequence and influenced by position and secondary structure.

Journal Article (Journal Article)

The sequence of the cloned Tetrahymena ribosomal RNA intervening sequence (IVS) was altered at the site to which circularization normally occurs. The alterations caused circularization to shift to other sites, usually a nearby position which followed three pyrimidines. While a tripyrimidine sequence was the major determinant of a circularization site, both location of a sequence and local secondary structure may influence the use of that sequence. For some constructs circularization appeared to occur at the position following the 5' G, the nucleotide added to the IVS during its excision. Portions of the internal guide sequence (IGS), proposed to interact with the 3'exon were deleted without preventing exon ligation. Thus if the IGS-3'exon interaction exists, it is not essential for splicing in vitro.

Full Text

Duke Authors

Cited Authors

  • Been, MD; Cech, TR

Published Date

  • December 9, 1985

Published In

Volume / Issue

  • 13 / 23

Start / End Page

  • 8389 - 8408

PubMed ID

  • 4080546

Pubmed Central ID

  • PMC322141

International Standard Serial Number (ISSN)

  • 0305-1048

Digital Object Identifier (DOI)

  • 10.1093/nar/13.23.8389


  • eng

Conference Location

  • England