Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex.
Publication
, Journal Article
Been, MD; Champoux, JJ
Published in: Nucleic Acids Res
December 20, 1980
The DNA nicking-closing enzyme (type I topoisomerase) from rat liver nuclei breaks single-stranded DNA. The broken strand contains a 5'-hydroxyl and tightly bound protein. The stability of this protein-DNA complex to high salt, alkali and detergent suggests a covalent linkage between the DNA and the enzyme. The observed breakage of single-stranded DNA occurs at neutral pH prior to treatment with alkali or detergent, indicating that the breakage may be the result of an interrupted nicking and closing cycle. The resulting covalent complex could represent a reaction intermediate in the overall nicking-closing reaction.
Duke Scholars
Published In
Nucleic Acids Res
DOI
ISSN
0305-1048
Publication Date
December 20, 1980
Volume
8
Issue
24
Start / End Page
6129 / 6142
Location
England
Related Subject Headings
- Ultracentrifugation
- Rats
- Protein Binding
- Liver
- Kinetics
- Developmental Biology
- DNA, Viral
- DNA, Single-Stranded
- DNA Topoisomerases, Type I
- Bacteriophage phi X 174
Citation
APA
Chicago
ICMJE
MLA
NLM
Been, M. D., & Champoux, J. J. (1980). Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex. Nucleic Acids Res, 8(24), 6129–6142. https://doi.org/10.1093/nar/8.24.6129
Been, M. D., and J. J. Champoux. “Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex.” Nucleic Acids Res 8, no. 24 (December 20, 1980): 6129–42. https://doi.org/10.1093/nar/8.24.6129.
Been MD, Champoux JJ. Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex. Nucleic Acids Res. 1980 Dec 20;8(24):6129–42.
Been, M. D., and J. J. Champoux. “Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex.” Nucleic Acids Res, vol. 8, no. 24, Dec. 1980, pp. 6129–42. Pubmed, doi:10.1093/nar/8.24.6129.
Been MD, Champoux JJ. Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex. Nucleic Acids Res. 1980 Dec 20;8(24):6129–6142.
Published In
Nucleic Acids Res
DOI
ISSN
0305-1048
Publication Date
December 20, 1980
Volume
8
Issue
24
Start / End Page
6129 / 6142
Location
England
Related Subject Headings
- Ultracentrifugation
- Rats
- Protein Binding
- Liver
- Kinetics
- Developmental Biology
- DNA, Viral
- DNA, Single-Stranded
- DNA Topoisomerases, Type I
- Bacteriophage phi X 174