Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex.

Journal Article (Journal Article)

The DNA nicking-closing enzyme (type I topoisomerase) from rat liver nuclei breaks single-stranded DNA. The broken strand contains a 5'-hydroxyl and tightly bound protein. The stability of this protein-DNA complex to high salt, alkali and detergent suggests a covalent linkage between the DNA and the enzyme. The observed breakage of single-stranded DNA occurs at neutral pH prior to treatment with alkali or detergent, indicating that the breakage may be the result of an interrupted nicking and closing cycle. The resulting covalent complex could represent a reaction intermediate in the overall nicking-closing reaction.

Full Text

Duke Authors

Cited Authors

  • Been, MD; Champoux, JJ

Published Date

  • December 20, 1980

Published In

Volume / Issue

  • 8 / 24

Start / End Page

  • 6129 - 6142

PubMed ID

  • 6258163

Pubmed Central ID

  • PMC328077

International Standard Serial Number (ISSN)

  • 0305-1048

Digital Object Identifier (DOI)

  • 10.1093/nar/8.24.6129


  • eng

Conference Location

  • England