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Conversion of protein farnesyltransferase to a geranylgeranyltransferase.

Publication ,  Journal Article
Terry, KL; Casey, PJ; Beese, LS
Published in: Biochemistry
August 15, 2006

Posttranslational modifications are essential for the proper function of a number of proteins in the cell. One such modification, the covalent attachment of a single isoprenoid lipid (prenylation), is carried out by the CaaX prenyltransferases, protein farnesyltransferase (FTase) and protein geranylgeranyltransferase type-I (GGTase-I). Substrate proteins of these two enzymes are involved in a variety of cellular functions but are largely associated with signal transduction. These modified proteins include members of the Ras superfamily, heterotrimeric G-proteins, centromeric proteins, and a number of proteins involved in nuclear integrity. Although FTase and GGTase-I are highly homologous, they are quite selective for their substrates, particularly for their isoprenoid diphosphate substrates, FPP and GGPP, respectively. Here, we present both crystallographic and kinetic analyses of mutants designed to explore this isoprenoid specificity and demonstrate that this specificity is dependent upon two enzyme residues in the beta subunits of the enzymes, W102beta and Y365beta in FTase (T49beta and F324beta, respectively, in GGTase-I).

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Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

August 15, 2006

Volume

45

Issue

32

Start / End Page

9746 / 9755

Location

United States

Related Subject Headings

  • Substrate Specificity
  • Protein Prenylation
  • Peptides
  • Mutation
  • Models, Molecular
  • Humans
  • Farnesyltranstransferase
  • Crystallography, X-Ray
  • Biochemistry & Molecular Biology
  • Binding Sites
 

Citation

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Terry, K. L., Casey, P. J., & Beese, L. S. (2006). Conversion of protein farnesyltransferase to a geranylgeranyltransferase. Biochemistry, 45(32), 9746–9755. https://doi.org/10.1021/bi060295e
Terry, Kimberly L., Patrick J. Casey, and Lorena S. Beese. “Conversion of protein farnesyltransferase to a geranylgeranyltransferase.Biochemistry 45, no. 32 (August 15, 2006): 9746–55. https://doi.org/10.1021/bi060295e.
Terry KL, Casey PJ, Beese LS. Conversion of protein farnesyltransferase to a geranylgeranyltransferase. Biochemistry. 2006 Aug 15;45(32):9746–55.
Terry, Kimberly L., et al. “Conversion of protein farnesyltransferase to a geranylgeranyltransferase.Biochemistry, vol. 45, no. 32, Aug. 2006, pp. 9746–55. Pubmed, doi:10.1021/bi060295e.
Terry KL, Casey PJ, Beese LS. Conversion of protein farnesyltransferase to a geranylgeranyltransferase. Biochemistry. 2006 Aug 15;45(32):9746–9755.
Journal cover image

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

August 15, 2006

Volume

45

Issue

32

Start / End Page

9746 / 9755

Location

United States

Related Subject Headings

  • Substrate Specificity
  • Protein Prenylation
  • Peptides
  • Mutation
  • Models, Molecular
  • Humans
  • Farnesyltranstransferase
  • Crystallography, X-Ray
  • Biochemistry & Molecular Biology
  • Binding Sites