Contact x-ray microscopy. A new technique for imaging cellular fine structure.

Journal Article (Journal Article)

Contact x-ray microscopy potentially allows living, wet cells to be visualized at a resolution of up to 100 A. Furthermore, differential absorption by specific elements permits the study of the distribution of those elements in biological specimens. In contact x-ray microscopy, soft x-rays (10 A to 100 A) pass through a biological sample and expose an underlying x-ray sensitive polymer (resist), producing an image that reflects the photon absorbance within the specimen. The high penetrating power of soft x-ray enables images to be obtained from specimens up to several microns thick. In this paper, the technique is described, some of the areas currently under study are considered, and biological examples of the use of contact x-ray microscopy are given.

Full Text

Duke Authors

Cited Authors

  • Beese, L; Feder, R; Sayre, D

Published Date

  • January 1, 1986

Published In

Volume / Issue

  • 49 / 1

Start / End Page

  • 259 - 268

PubMed ID

  • 3955174

Pubmed Central ID

  • PMC1329631

International Standard Serial Number (ISSN)

  • 0006-3495

Digital Object Identifier (DOI)

  • 10.1016/S0006-3495(86)83639-6


  • eng

Conference Location

  • United States