Ank3-dependent SVZ niche assembly is required for the continued production of new neurons.
Journal Article (Journal Article)
The rodent subventricular/subependymal zone (SVZ/SEZ) houses neural stem cells (NSCs) that generate olfactory bulb interneurons. It is unclear how the SVZ environment sustains neuronal production into adulthood. We discovered that the adapter molecule Ankyrin-3 (Ank3) is specifically upregulated in ventricular progenitors destined to become ependymal cells, but not in NSCs, and is required for SVZ niche assembly through progenitor lateral adhesion. Furthermore, we found that Ank3 expression is controlled by Foxj1, a transcriptional regulator of multicilia formation, and genetic deletion of this pathway led to complete loss of SVZ niche structure. Interestingly, radial glia continued to transition into postnatal NSCs without this niche. However, inducible deletion of Foxj1-Ank3 from mature SVZ ependyma resulted in dramatic depletion of neurogenesis. Targeting a pathway regulating ependymal organization/assembly and showing its requirement for new neuron production, our results have important implications for environmental control of adult neurogenesis and harvesting NSCs for replacement therapy.
Full Text
Duke Authors
Cited Authors
- Paez-Gonzalez, P; Abdi, K; Luciano, D; Liu, Y; Soriano-Navarro, M; Rawlins, E; Bennett, V; Garcia-Verdugo, JM; Kuo, CT
Published Date
- July 14, 2011
Published In
Volume / Issue
- 71 / 1
Start / End Page
- 61 - 75
PubMed ID
- 21745638
Pubmed Central ID
- PMC3134799
Electronic International Standard Serial Number (EISSN)
- 1097-4199
Digital Object Identifier (DOI)
- 10.1016/j.neuron.2011.05.029
Language
- eng
Conference Location
- United States