L1-dependent neuritogenesis involves ankyrinB that mediates L1-CAM coupling with retrograde actin flow.
Published
Journal Article
The cell adhesion molecule L1 (L1-CAM) plays critical roles in neurite growth. Its cytoplasmic domain (L1CD) binds to ankyrins that associate with the spectrin-actin network. This paper demonstrates that L1-CAM interactions with ankyrinB (but not with ankyrinG) are involved in the initial formation of neurites. In the membranous protrusions surrounding the soma before neuritogenesis, filamentous actin (F-actin) and ankyrinB continuously move toward the soma (retrograde flow). Bead-tracking experiments show that ankyrinB mediates L1-CAM coupling with retrograde F-actin flow in these perisomatic structures. Ligation of the L1-CAM ectodomain by an immobile substrate induces L1CD-ankyrinB binding and the formation of stationary ankyrinB clusters. Neurite initiation preferentially occurs at the site of these clusters. In contrast, ankyrinB is involved neither in L1-CAM coupling with F-actin flow in growth cones nor in L1-based neurite elongation. Our results indicate that ankyrinB promotes neurite initiation by acting as a component of the clutch module that transmits traction force generated by F-actin flow to the extracellular substrate via L1-CAM.
Full Text
Duke Authors
Cited Authors
- Nishimura, K; Yoshihara, F; Tojima, T; Ooashi, N; Yoon, W; Mikoshiba, K; Bennett, V; Kamiguchi, H
Published Date
- December 8, 2003
Published In
Volume / Issue
- 163 / 5
Start / End Page
- 1077 - 1088
PubMed ID
- 14657231
Pubmed Central ID
- 14657231
International Standard Serial Number (ISSN)
- 0021-9525
Digital Object Identifier (DOI)
- 10.1083/jcb.200303060
Language
- eng
Conference Location
- United States