Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy.
Journal Article (Journal Article)
Glioblastomas shed large quantities of small, membrane-bound microvesicles into the circulation. Although these hold promise as potential biomarkers of therapeutic response, their identification and quantification remain challenging. Here, we describe a highly sensitive and rapid analytical technique for profiling circulating microvesicles directly from blood samples of patients with glioblastoma. Microvesicles, introduced onto a dedicated microfluidic chip, are labeled with target-specific magnetic nanoparticles and detected by a miniaturized nuclear magnetic resonance system. Compared with current methods, this integrated system has a much higher detection sensitivity and can differentiate glioblastoma multiforme (GBM) microvesicles from nontumor host cell-derived microvesicles. We also show that circulating GBM microvesicles can be used to analyze primary tumor mutations and as a predictive metric of treatment-induced changes. This platform could provide both an early indicator of drug efficacy and a potential molecular stratifier for human clinical trials.
Full Text
Duke Authors
Cited Authors
- Shao, H; Chung, J; Balaj, L; Charest, A; Bigner, DD; Carter, BS; Hochberg, FH; Breakefield, XO; Weissleder, R; Lee, H
Published Date
- December 2012
Published In
Volume / Issue
- 18 / 12
Start / End Page
- 1835 - 1840
PubMed ID
- 23142818
Pubmed Central ID
- PMC3518564
Electronic International Standard Serial Number (EISSN)
- 1546-170X
Digital Object Identifier (DOI)
- 10.1038/nm.2994
Language
- eng
Conference Location
- United States