Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy.

Published

Journal Article

Glioblastomas shed large quantities of small, membrane-bound microvesicles into the circulation. Although these hold promise as potential biomarkers of therapeutic response, their identification and quantification remain challenging. Here, we describe a highly sensitive and rapid analytical technique for profiling circulating microvesicles directly from blood samples of patients with glioblastoma. Microvesicles, introduced onto a dedicated microfluidic chip, are labeled with target-specific magnetic nanoparticles and detected by a miniaturized nuclear magnetic resonance system. Compared with current methods, this integrated system has a much higher detection sensitivity and can differentiate glioblastoma multiforme (GBM) microvesicles from nontumor host cell-derived microvesicles. We also show that circulating GBM microvesicles can be used to analyze primary tumor mutations and as a predictive metric of treatment-induced changes. This platform could provide both an early indicator of drug efficacy and a potential molecular stratifier for human clinical trials.

Full Text

Duke Authors

Cited Authors

  • Shao, H; Chung, J; Balaj, L; Charest, A; Bigner, DD; Carter, BS; Hochberg, FH; Breakefield, XO; Weissleder, R; Lee, H

Published Date

  • December 2012

Published In

Volume / Issue

  • 18 / 12

Start / End Page

  • 1835 - 1840

PubMed ID

  • 23142818

Pubmed Central ID

  • 23142818

Electronic International Standard Serial Number (EISSN)

  • 1546-170X

Digital Object Identifier (DOI)

  • 10.1038/nm.2994

Language

  • eng

Conference Location

  • United States