Skip to main content

Role of the Fas-signaling pathway in photoreceptor neuroprotection.

Publication ,  Journal Article
Zacks, DN; Boehlke, C; Richards, A-L; Zheng, Q-D
Published in: Arch Ophthalmol
October 2007

OBJECTIVE: To determine whether inhibiting the Fas proapoptosis pathway will result in increased photoreceptor survival after separation of the retina from the retinal pigment epithelium (RPE). METHODS: Retina/RPE separation was induced in rat and mouse eyes by the subretinal injection of hyaluronic acid, 1%. Fas-pathway signaling was inhibited by the concomitant injection of a Fas receptor-neutralizing antibody, small inhibitory RNA against the Fas-receptor transcript (siFAS), or the use of the Fas-receptor defective mouse strain LPR. Indices of photoreceptor death included terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining, cell counts, and retinal thickness measurements. Retinas were immunostained with antibodies against rhodopsin and cone opsin to evaluate rod and cone photopigment production, respectively. RESULTS: Inhibition of Fas signaling using Fas receptor-neutralizing antibody, siFas, or LPR mice resulted in a significant reduction in the number of TUNEL-positive photoreceptor cells as well as in a significant preservation of outer nuclear layer cell counts and thickness as compared with retina/RPE separation in eyes with intact Fas signaling. Fas-pathway inhibition resulted in preservation of both rhodopsin- and cone opsin-positive cells. CONCLUSIONS: Inhibition of the Fas proapoptosis pathway results in significant photoreceptor preservation after retinal separation from the RPE. CLINICAL RELEVANCE: Fas-pathway inhibition might serve as a novel mechanism for preserving photoreceptor cells during retinal disease.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Arch Ophthalmol

DOI

ISSN

0003-9950

Publication Date

October 2007

Volume

125

Issue

10

Start / End Page

1389 / 1395

Location

United States

Related Subject Headings

  • fas Receptor
  • Signal Transduction
  • Rod Opsins
  • Rhodopsin
  • Reverse Transcriptase Polymerase Chain Reaction
  • Retinal Detachment
  • Rats, Inbred BN
  • Rats
  • RNA, Small Interfering
  • RNA
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zacks, D. N., Boehlke, C., Richards, A.-L., & Zheng, Q.-D. (2007). Role of the Fas-signaling pathway in photoreceptor neuroprotection. Arch Ophthalmol, 125(10), 1389–1395. https://doi.org/10.1001/archopht.125.10.1389
Zacks, David N., Christopher Boehlke, Ayo-Lynn Richards, and Qiong-Duan Zheng. “Role of the Fas-signaling pathway in photoreceptor neuroprotection.Arch Ophthalmol 125, no. 10 (October 2007): 1389–95. https://doi.org/10.1001/archopht.125.10.1389.
Zacks DN, Boehlke C, Richards A-L, Zheng Q-D. Role of the Fas-signaling pathway in photoreceptor neuroprotection. Arch Ophthalmol. 2007 Oct;125(10):1389–95.
Zacks, David N., et al. “Role of the Fas-signaling pathway in photoreceptor neuroprotection.Arch Ophthalmol, vol. 125, no. 10, Oct. 2007, pp. 1389–95. Pubmed, doi:10.1001/archopht.125.10.1389.
Zacks DN, Boehlke C, Richards A-L, Zheng Q-D. Role of the Fas-signaling pathway in photoreceptor neuroprotection. Arch Ophthalmol. 2007 Oct;125(10):1389–1395.

Published In

Arch Ophthalmol

DOI

ISSN

0003-9950

Publication Date

October 2007

Volume

125

Issue

10

Start / End Page

1389 / 1395

Location

United States

Related Subject Headings

  • fas Receptor
  • Signal Transduction
  • Rod Opsins
  • Rhodopsin
  • Reverse Transcriptase Polymerase Chain Reaction
  • Retinal Detachment
  • Rats, Inbred BN
  • Rats
  • RNA, Small Interfering
  • RNA